Related Experiment Video

Updated: Jul 12, 2025

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
08:45

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Published on: December 28, 2017

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The regulatory subunits of CK2 complex mediate DNA damage response and virulence in Candida Glabrata

Qi Ni1, Xianwei Wu2, Tongxuan Su1

  • 1Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, No.197 Ruijin ER Road, Shanghai, 200025, China.

BMC Microbiology
|October 27, 2023
PubMed
Abstract

Insights

Regulatory subunits CgCKB1 and CgCKB2 are vital for Candida glabrata

Area of Science:

  • Microbiology
  • Molecular Biology
  • Mycology

Background:

  • Candida glabrata is a prevalent opportunistic pathogen known for drug resistance.
  • C. glabrata evades host immunity, leading to persistent infections.
  • Mechanisms of C. glabrata's DNA damage response are not fully understood.

Purpose of the Study:

  • Investigate the role of CgCK2 regulatory subunits (CgCkb1, CgCkb2) in DNA damage response.
  • Determine the impact of CgCKB1 and CgCKB2 on C. glabrata virulence and survival.

Main Methods:

  • Gene deletion studies (Δckb1, Δckb2 strains).
  • Analysis of cellular apoptosis and DNA breaks.
  • Phagocytosis survival assays.
  • Murine models of invasive candidiasis.
  • Global transcriptional profiling.

Main Results:

  • Deletion of CgCKB1 or CgCKB2 increased apoptosis and DNA breaks.
  • Δckb1 and Δckb2 strains showed reduced survival after phagocytosis.
  • Disruption of CgCKB1/CgCKB2 attenuated virulence in vivo.
  • CgCkb1 and CgCkb2 are involved in cell cycle resumption and genomic stability.

Conclusions:

  • DNA damage response is critical for C. glabrata survival in macrophages and virulence.
  • CgCKB1 and CgCKB2 are key regulators of DNA damage response and virulence.
  • Findings enhance understanding of candidiasis pathogenicity and suggest therapeutic targets.
Keywords:
Candida glabrataCell cycleDNA damageMacrophageVirulence

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