Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Open Angle Glaucoma: Treatment01:27

Open Angle Glaucoma: Treatment

459
In open-angle glaucoma, the iridocorneal angle remains open, but the trabecular meshwork becomes stiff, slowing down the outflow of aqueous humor. This causes a buildup of aqueous humor in the anterior chamber, leading to a sudden increase in intraocular pressure. The treatment for open-angle glaucoma focuses on reducing the elevated intraocular pressure by either decreasing the secretion of aqueous humor or increasing its outflow.
Drugs such as carbonic anhydrase inhibitors, α2- and...
459
Glaucoma: Overview01:25

Glaucoma: Overview

599
Glaucoma is an eye condition characterized by increased intraocular pressure that damages the retina and optic nerve, leading to irreversible blindness if left untreated. The human eye has various components, including the cornea, iris, pupil, lens, and optic nerve. Aqueous humor is secreted by the epithelium of the ciliary body in the posterior chamber and flows through the trabecular meshwork and canal of Schlemm, maintaining normal intraocular pressure. The trabecular meshwork and the canal...
599
Angle Closure Glaucoma: Treatment01:28

Angle Closure Glaucoma: Treatment

536
Angle-closure glaucoma, or closed-angle glaucoma, is an eye condition where the iris bulges out and blocks the iridocorneal angle, resulting in a buildup of aqueous humor and increased intraocular pressure. Immediate medical attention is necessary due to the sudden onset of symptoms. The treatment for angle-closure glaucoma includes short-term and long-term approaches. Short-term treatment involves using eye drops like pilocarpine to lower intraocular pressure by increasing aqueous humor...
536

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Choroidal vascularity profiles in diabetic macular oedema subtypes: a swept-source OCT angiography study.

Eye (London, England)·2026
Same author

Response to: Comment on: "Epiretinal membrane formation and incomplete separation of the posterior hyaloid membrane".

Eye (London, England)·2026
Same author

Impact of Laser Photocoagulation for Peripheral Retinal Degeneration and Breaks on Macular Structure and Function.

Current eye research·2026
Same author

Comparative Performance Evaluation of Large Language Models and Human Teachers in Answering Optometry Questions from Medical Undergraduates.

Journal of medical education and curricular development·2026
Same author

Blood Cell-Derived Inflammatory Indices in Diabetic Macular Edema: Clinical Significance and Prognostic Relevance.

Biomedicines·2025
Same author

Targeting RPE Senescence Via Suppressing IL-6/IL-6R Signaling for Treating Retinal Degenerative Diseases.

Investigative ophthalmology & visual science·2025

Related Experiment Video

Updated: Jul 12, 2025

A Mouse Model of Retinal Ischemia-Reperfusion Injury Through Elevation of Intraocular Pressure
07:37

A Mouse Model of Retinal Ischemia-Reperfusion Injury Through Elevation of Intraocular Pressure

Published on: July 14, 2016

15.1K

HIF-1α Reduction by Lowering Intraocular Pressure Alleviated Retinal Neovascularization.

Ziqi Yang1, Biyan Ni1, Tian Zhou1

  • 1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou 510060, China.

Biomolecules
|October 28, 2023
PubMed
Summary
This summary is machine-generated.

Larger needles used for eye injections unexpectedly reduced retinal neovascularization by lowering intraocular pressure. This finding suggests that reducing intraocular pressure may be a new treatment for blinding eye diseases.

Keywords:
hypoxia-inducible factor-1 alpha (HIF-1α)intraocular pressure (IOP)intravitreal injectionoxygen-induced retinopathy (OIR)retinal neovascularization

More Related Videos

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents
09:28

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents

Published on: September 16, 2020

8.5K
Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
07:32

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina

Published on: June 23, 2014

14.5K

Related Experiment Videos

Last Updated: Jul 12, 2025

A Mouse Model of Retinal Ischemia-Reperfusion Injury Through Elevation of Intraocular Pressure
07:37

A Mouse Model of Retinal Ischemia-Reperfusion Injury Through Elevation of Intraocular Pressure

Published on: July 14, 2016

15.1K
Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents
09:28

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents

Published on: September 16, 2020

8.5K
Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
07:32

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina

Published on: June 23, 2014

14.5K

Area of Science:

  • Ophthalmology
  • Angiogenesis Research
  • Vascular Biology

Background:

  • Retinal neovascularization, driven by hypoxia, is a major cause of blindness.
  • Oxygen-induced retinopathy (OIR) models are crucial for studying anti-angiogenic therapies.
  • Intravitreal injections are standard for delivering treatments in OIR models.

Purpose of the Study:

  • To investigate the impact of different intravitreal puncture sizes on retinal neovascularization.
  • To explore the underlying mechanisms of the observed anti-angiogenic effects.
  • To evaluate the potential of intraocular pressure (IOP) reduction as a therapeutic strategy.

Main Methods:

  • Intravitreal punctures of varying needle sizes (0.5 mm, 0.3 mm, 0.21 mm) were performed in OIR mice.
  • Retinal neovascularization, vascular leakage, and retinal vasculature were assessed.
  • Intraocular pressure (IOP), oxygen partial pressure (pO2), and Hypoxia-inducible factor 1-alpha (Hif1a) expression were measured.
  • The efficacy of IOP-lowering drugs (Travatan, Azarga) was evaluated.

Main Results:

  • A 0.5 mm needle puncture significantly suppressed pathological angiogenesis and vascular leakage compared to smaller needles.
  • The 0.5 mm puncture led to a marked reduction in IOP, improving oxygenation and decreasing Hif1a expression.
  • IOP-lowering drugs demonstrated potent anti-angiogenesis effects by alleviating hypoxia.
  • Reduced IOP was directly linked to suppressed HIF-1α-mediated retinal neovascularization.

Conclusions:

  • Intravitreal injection needle size significantly influences retinal neovascularization.
  • A larger puncture (0.5 mm) induces acute IOP reduction, suppressing hypoxia and HIF-1α.
  • Lowering intraocular pressure is a promising therapeutic approach for retinal neovascular diseases.