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Related Concept Videos

Fertilization01:38

Fertilization

During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
Sperm Structure and Semen Composition01:22

Sperm Structure and Semen Composition

During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...

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Sperm Collection of Differential Quality Using Density Gradient Centrifugation
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Stain-Free Sperm Analysis and Selection for Intracytoplasmic Sperm Injection Complying with WHO Strict Normal

Yulia Michailov1,2, Luba Nemerovsky3,4, Yehudith Ghetler4

  • 1Obstetrics and Gynecology Department, Barzilai University Medical Center, Ashkelon 7830604, Israel.

Biomedicines
|October 28, 2023
PubMed
Summary

Quantitative phase microscopy (QPM) offers a label-free method for selecting sperm cells for intracytoplasmic sperm injection (ICSI). This non-invasive technique shows high repeatability and agrees with traditional methods, potentially improving ICSI success rates.

Keywords:
IVF ICSIWHO2021quantitative phase microscopy (QPM)sperm selection

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Area of Science:

  • Reproductive biology and assisted reproductive technologies
  • Microscopy and imaging techniques
  • Andrology and male infertility diagnostics

Background:

  • Current sperm selection for intracytoplasmic sperm injection (ICSI) relies on subjective assessment using bright field microscopy.
  • The World Health Organization (WHO) 2021 criteria provide objective standards for sperm morphology assessment.
  • There is a need for objective, label-free methods to improve sperm selection accuracy in ICSI.

Purpose of the Study:

  • To evaluate a novel quantitative phase microscopy (QPM) system for label-free sperm cell selection.
  • To compare QPM measurements with traditional bright field microscopy of stained sperm cells.
  • To assess the accuracy of QPM in identifying sperm cells meeting WHO 2021 criteria.

Main Methods:

  • Multi-center study involving 73 patients undergoing in vitro fertilization (IVF).
  • Sperm cells were selected by embryologists using bright field microscopy and then imaged with QPM.
  • Sperm cells were subsequently stained and measured manually by blinded embryologists for comparison.

Main Results:

  • QPM measurements showed good agreement with traditional methods for head length, head width, and length-width ratio.
  • The repeatability of measurements was significantly higher with QPM compared to bright field microscopy.
  • Only 19% of subjectively selected 'normal' sperm cells met the WHO 2021 criteria, highlighting selection challenges.

Conclusions:

  • Quantitative phase microscopy (QPM) is a non-toxic, non-invasive tool for sperm selection in ICSI.
  • QPM offers improved measurement repeatability and accuracy compared to traditional methods.
  • This technology has the potential to enhance the clinical effectiveness of ICSI by enabling more precise sperm selection.