Related Experiment Video
Updated: Jul 2, 2026
12:17
Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells
Published on: October 28, 2009
10.8K
Clonal and Scalable Endothelial Progenitor Cell Lines from Human Pluripotent Stem Cells.
Jieun Lee1, Hal Sternberg1, Paola A Bignone1
1AgeX Therapeutics, Inc., 1101 Marina Village Parkway, Alameda, CA 94501, USA.
Biomedicines
|October 28, 2023
Summary
Human embryonic stem cell-derived endothelial progenitor cells (eEPCs) offer a scalable source for treating cardiovascular disease. These cells maintain stable angiogenic properties, showing promise for therapeutic applications.
Area of Science:
- Stem cell biology
- Cardiovascular research
- Regenerative medicine
Background:
- Human pluripotent stem cells (hPSCs) are a potential source of endothelial cells for treating cardiovascular and ischemic diseases.
- Developing renewable and scalable cell sources is crucial for therapeutic applications.
Purpose of the Study:
- To derive and characterize distinct clonal embryonic endothelial progenitor cell (eEPC) lines from human embryonic stem cells (hESCs).
- To assess the potential of these eEPCs as a scalable therapeutic cell source for cardiovascular disease.
Main Methods:
- Partial differentiation of hESCs (ESI-017) to generate candidate cultures for eEPC cloning.
- Comprehensive characterization including transcriptomic analysis, cell surface marker expression, immunocytochemistry, and functional vascular network assays.
- Comparison of eEPC transcriptomes with adult endothelial and non-endothelial cells.
Main Results:
- Derived distinct clonal eEPC lines with functional endothelial properties and strong transcriptomic similarity to adult endothelial cells.
- Identified a novel embryonic gene expression pattern distinguishing eEPCs from adult endothelium.
- Demonstrated scalability up to 80 population doublings and stable long-term expansion (>50 pd) with maintained angiogenic properties.
Conclusions:
- hESC-derived clonal eEPC lines represent a promising, scalable source of therapeutic cells and cell products for cardiovascular disease.
- These eEPC lines are suitable for further preclinical studies and therapeutic intervention development.
More Related Videos
Related Concept Videos
01:33Adult Stem Cells
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...
01:10Zygotic Development And Stem Cell Formation
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
01:12Renewal of Skin Epidermal Stem Cells
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
01:06Multipotency and Niche of Bulge Stem Cell
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
01:13Cell Diversity
The concept of a cell started with microscopic observations of dead cork tissue by Robert Hooke in 1665. Hooke coined the term "cell" based on the resemblance of the small subdivisions in the cork to the rooms that monks inhabited, called cells. About ten years later, Antonie van Leeuwenhoek became the first person to observe the living and moving cells under a microscope. In the century that followed, the theory that cells represented the basic unit of life developed.
Multicellular organisms...
Multicellular organisms...
01:24Cells of the Epidermis
The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum.
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...