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Related Experiment Videos

Adipocyte development in primary rat cell cultures: a scanning electron microscopy study.

R L Richardson, G J Hausman, D R Campion

    The Anatomical Record
    |November 1, 1986
    PubMed
    Summary

    Scanning electron microscopy (SEM) and X-ray microprobe analysis (XRMA) reveal adipocyte development in rat stromal-vascular cells. Different substrates did not affect cell morphology, actin cables, or lipid accumulation.

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    Area of Science:

    • Cell Biology
    • Biophysics
    • Histology

    Background:

    • Stromal-vascular (SV) cells are precursors to adipocytes.
    • Understanding adipocyte differentiation in vitro is crucial for metabolic research.
    • Scanning electron microscopy (SEM) offers high-resolution imaging of cellular structures.

    Purpose of the Study:

    • To investigate the morphology and differentiation of primary rat SV cells in vitro.
    • To evaluate the influence of different substrate coatings on adipocyte development.
    • To demonstrate the utility of SEM and X-ray microprobe analysis (XRMA) for cytochemical analysis.

    Main Methods:

    • Primary cultures of rat inguinal adipose tissue SV cells.
    • Cell culture on collagen-coated, fibronectin-coated, and uncoated glass coverslips.

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  • Fixation methods including glutaraldehyde/osmium tetroxide and freeze-drying.
  • X-ray microprobe analysis (XRMA) for inosine diphosphatase detection.

    • Main Results:

    • Adipocyte morphology was consistent across all tested substrates.
    • Actin cables were observed in developing adipocytes by day 2.
    • No significant differences in actin structure, cell shape, or lipid accumulation were noted between substrates.
    • Proliferation of non-adipogenic stromal cells into multilayers occurred by day 9.
    • XRMA confirmed inosine diphosphatase localization within the Golgi apparatus of developing adipocytes.

    Conclusions:

    • SEM and XRMA are effective tools for characterizing adipocyte morphology and cytochemistry in vitro.
    • Substrate coatings do not significantly alter the early differentiation of rat SV cells.
    • Primary cultured rat SV cells exhibit distinct proliferative and adipogenic behaviors.