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Network Pharmacology and Bioinformatics Study of Geniposide Regulating Oxidative Stress in Colorectal Cancer

Yingzi Wu1, Jinhai Luo1, Baojun Xu1

  • 1Guangdong Provincial Key Laboratory IRADS, Department of Life Sciences, BNU-HKBU United International College, Zhuhai 519087, China.

International Journal of Molecular Sciences
|October 28, 2023
PubMed

Insights

Geniposide combats colorectal cancer (CRC) by regulating oxidative stress. This study identified key genes and pathways involved, revealing geniposide

Area of Science:

  • Integrative oncology and molecular pharmacology.
  • Application of network pharmacology and bioinformatics in cancer research.

Background:

  • Colorectal cancer (CRC) is a significant global health concern.
  • Oxidative stress plays a critical role in CRC development and progression.
  • Geniposide, a natural compound, shows potential therapeutic effects, but its mechanism in CRC is not fully elucidated.

Purpose of the Study:

  • To elucidate the mechanism by which geniposide regulates oxidative stress in colorectal cancer.
  • To identify key molecular targets and pathways involved in geniposide's anti-CRC effects.
  • To provide a scientific basis for geniposide as a potential therapeutic agent for CRC.

Main Methods:

  • Network pharmacology and bioinformatics analysis to identify geniposide targets, CRC targets, and oxidative stress-related targets.
  • Protein-protein interaction (PPI) network analysis to determine hub genes.
  • Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis.
  • Molecular docking, GEPIA, HPA, and starBase databases for hub gene verification.

Main Results:

  • Five hub genes (IL1B, GSK3B, NOS3, RELA, CDK4) were identified as crucial for geniposide's action.
  • GO analysis indicated involvement in processes like response to temperature stimulus and nitric oxide metabolism.
  • KEGG analysis highlighted significant pathways including PI3K-Akt, IL-17, p53, NF-κB, and NOD-like receptor signaling.
  • Molecular docking confirmed good binding activity between geniposide and the identified hub genes.

Conclusions:

  • Geniposide effectively regulates oxidative stress in colorectal cancer.
  • The anti-cancer effects of geniposide are linked to its modulation of multiple biological processes and signaling pathways.
  • Induction of oxidative stress is a potential mechanism underlying geniposide's anti-recurrence and anti-metastasis effects in CRC.
Keywords:
CRCbioinformaticsgeniposidemolecular dockingnetwork pharmacologyoxidative stress

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