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Related Concept Videos

Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

236
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
236
Methods for Studying Drug Absorption: In situ01:09

Methods for Studying Drug Absorption: In situ

244
In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
244

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Updated: Jul 12, 2025

Modeling and Simulations of Olfactory Drug Delivery with Passive and Active Controls of Nasally Inhaled Pharmaceutical Aerosols
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The Path from Nasal Tissue to Nasal Mucosa on Chip: Part 2-Advanced Microfluidic Nasal In Vitro Model for Drug

Eugen Viktor Koch1,2, Sebastian Bendas2,3, Kristina Nehlsen4

  • 1Institute of Microtechnology, TU Braunschweig, Alte Salzdahlumer Str. 203, 38124 Braunschweig, Germany.

Pharmaceutics
|October 28, 2023
PubMed
Summary

Researchers developed a novel nasal mucosa on a chip platform for drug delivery research. This biomimetic system enhances cilia formation and mucus distribution, aiding preclinical drug testing.

Keywords:
microfluidicsmucociliary clearancenasal drug administrationnasal mucosaorgan on chippermeation test

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Area of Science:

  • Biomedical Engineering
  • Pharmacology
  • Drug Delivery Systems

Background:

  • The nasal mucosa offers a promising route for systemic drug administration due to its accessibility and vascularization.
  • Physiological barriers, such as mucociliary clearance, pose significant challenges for drug candidates in nasal drug delivery.
  • Effective preclinical testing models are crucial for evaluating drug permeability and efficacy through the nasal mucosa.

Purpose of the Study:

  • To develop and validate a novel microfluidic chip platform mimicking the human nasal mucosa for drug permeation studies.
  • To assess the physiological relevance of the microfluidic nasal mucosa model in terms of mucus layer formation and ciliary function.
  • To evaluate the utility of the platform for determining drug permeation coefficients in a preclinical setting.

Main Methods:

  • Microfabrication of a biomimetic glass chip with controlled bidirectional airflow to simulate physiological shear stress.
  • Development of a membrane transfer technique for pre-cultivating an immortalized porcine nasal epithelial cell layer.
  • Dynamic cultivation within the microfluidic chip and assessment of mucus distribution and cilia formation.
  • Utilizing microparticle image velocimetry to record ciliary transport mechanisms.
  • Conducting permeation studies using FITC-dextran 4000 to determine the permeation coefficient.

Main Results:

  • Dynamic cultivation in the microfluidic chip resulted in homogenous mucus film distribution and significantly increased cilia formation compared to static conditions.
  • Successful recording of the ciliary transport mechanism was achieved using microparticle image velocimetry.
  • The nasal mucosa on a chip model demonstrated suitability for permeation studies, yielding a permeation coefficient comparable to established in vitro and in vivo models.
  • The developed platform provides a more physiologically relevant environment for studying nasal drug absorption.

Conclusions:

  • The novel microfluidic nasal mucosa platform effectively replicates key physiological features of the nasal epithelium, including mucus layer and ciliary function.
  • This 'nasal mucosa on a chip' system serves as a valuable tool for preclinical drug permeation studies, offering a more accurate assessment of drug delivery potential.
  • The platform holds promise for future automation and application as a non-animal alternative for pharmaceutical research and development.