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PluriBAC: A Versatile Baculovirus-Based Modular System to Express Heterologous Genes in Different Biotechnological
Leslie C Amorós Morales1, Abril Marchesini1, Santiago M Gómez Bergna1
1Instituto de Biotecnología y Biología Molecular (IBBM, UNLP-CONICET), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Consejo Nacional de Investigaciones Científicas y Técnicas, La Plata B1900, Argentina.
Viruses
|October 28, 2023
Summary
The PluriBAC system streamlines the creation of recombinant baculoviruses for biotechnology. This versatile tool enables the development of novel insecticide and gene therapy vectors.
Area of Science:
- Biotechnology
- Molecular Virology
- Insect Pathology
Background:
- Baculoviruses, particularly Autographa californica nucleopolyhedrovirus (AcMNPV), are valuable insect-specific pathogens used in biotechnology for bio-input production.
- Improving technologies for generating recombinant baculoviruses is crucial for advancing their applications.
Purpose of the Study:
- To develop a versatile system for generating baculoviral transfer vectors.
- To demonstrate the utility of this system for creating reporter vectors and potential insecticide and gene therapy vectors.
Main Methods:
- Development of the PluriBAC system utilizing Golden Gate assembly technology with three insert entry levels.
- Design of vectors allowing combinations of multiple promoters, genes of interest, and terminator sequences.
- Generation and testing of recombinant AcMNPV baculoviruses.
Main Results:
- The PluriBAC system demonstrated versatility in combining various genetic elements.
- Recombinant AcMNPV generated using PluriBAC successfully infected *Spodoptera frugiperda* larvae.
- Recombinant budded virions (BV) effectively transduced tumor and normal cells in vitro and in vivo.
Conclusions:
- The PluriBAC system offers a flexible platform for generating baculoviral vectors.
- This system facilitates the creation of baculoviruses for applications in insect control and gene therapy.
- The developed system supports the generation of baculoviral reporter vectors for diverse biotechnological fields.
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