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Updated: Jul 12, 2025

Following Cell-fate in E. coli After Infection by Phage Lambda
Published on: October 14, 2011
The Dynamics of Synthesis and Localization of Jumbo Phage RNA Polymerases inside Infected Cells
Daria Antonova1, Viktoriia V Belousova1, Erik Zhivkoplias1
1Research Center of Nanobiotechnologies, Peter the Great St. Petersburg Polytechnic University, St. Petersburg 195251, Russia.
Abstract:
A nucleus-like structure composed of phage-encoded proteins and containing replicating viral DNA is formed in Pseudomonas aeruginosa cells infected by jumbo bacteriophage phiKZ. The PhiKZ genes are transcribed independently from host RNA polymerase (RNAP) by two RNAPs encoded by the phage. The virion RNAP (vRNAP) transcribes early viral genes and must be injected into the cell with phage DNA. The non-virion RNAP (nvRNAP) is composed of early gene products and transcribes late viral genes. In this work, the dynamics of phage RNAPs localization during phage phiKZ infection were studied. We provide direct evidence of PhiKZ vRNAP injection in infected cells and show that it is excluded from the phage nucleus. The nvRNAP is synthesized shortly after the onset of infection and localizes in the nucleus. We propose that spatial separation of two phage RNAPs allows coordinated expression of phage genes belonging to different temporal classes.
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