Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Xylem and Transpiration-driven Transport of Resources02:03

Xylem and Transpiration-driven Transport of Resources

23.9K
The xylem of vascular plants distributes water and dissolved minerals that are taken up by the roots to the rest of the plant. The cells that transport xylem sap are dead upon maturity, and the movement of xylem sap is a passive process.
23.9K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.3K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plant stem cell systems: Robust by design.

Developmental cell·2026
Same author

Multi-omics dissection of steviol glycoside synthesis reveals haplotype-linked specialization of UGT76G genes in Stevia rebaudiana.

The New phytologist·2026
Same author

Reference Genome Assembly of a Tetraploid Accession of the Tuber Crop Tropaeolum tuberosum.

Genome biology and evolution·2026
Same author

Decoding plant cell heterogeneity and dynamics across responses, development, to evolution with single-cell technologies.

Current opinion in plant biology·2026
Same author

An Open Dataset of Chinese Name-to-Gender Associations for Gender Prediction in Broad Scientific Research.

Scientific data·2025
Same author

Integration of single nucleus RNA-seq and bulk RNA-seq reveals gene regulatory networks for vascular connection between parasitic plants and host plants.

Journal of plant research·2025

Related Experiment Video

Updated: Jul 12, 2025

Isolation and Transcriptome Analysis of Plant Cell Types
08:53

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

1.5K

Analysis of Xylem Cells by Nucleus-Based Transcriptomics and Chromatin Profiling.

Dongbo Shi1,2,3, Laura Luzzietti4, Michael Nodine5

  • 1Department of Developmental Physiology, Centre for Organismal Studies (COS), Heidelberg University, Heidelberg, Germany. thseki@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)
|October 28, 2023
PubMed
Summary

Researchers developed new methods to analyze cell nuclei from plant xylem tissues. These techniques enable detailed studies of gene expression and chromatin structure in these specialized cells.

Keywords:
ATAC-seqFANSFlow cytometryNucleus sortingRNA-seqSMART-seq2

More Related Videos

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
10:03

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Published on: May 12, 2023

3.1K
An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
09:33

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis

Published on: June 19, 2014

13.2K

Related Experiment Videos

Last Updated: Jul 12, 2025

Isolation and Transcriptome Analysis of Plant Cell Types
08:53

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

1.5K
Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
10:03

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Published on: May 12, 2023

3.1K
An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
09:33

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis

Published on: June 19, 2014

13.2K

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Nuclei store critical cell state information via chromatin and RNA profiles.
  • High-throughput sequencing technologies now allow detailed analysis of these nuclear features.

Purpose of the Study:

  • To describe analytical pipelines for nucleus isolation and subsequent molecular profiling from differentiated xylem tissues.
  • To enable tissue-specific analysis of gene expression and chromatin structure.

Main Methods:

  • Nucleus isolation using fluorescence-activated nucleus sorting (FANS).
  • Transcriptome profiling via SMART-seq2.
  • Chromatin accessibility analysis using assay for transposase-accessible chromatin (ATAC)-seq.
  • Integration with tissue-specific nuclear fluorescent reporters.

Main Results:

  • Established pipelines for obtaining tissue-specific gene expression and chromatin structure data.
  • Demonstrated applicability across diverse cell types, tissues, and organs.
  • Xylem cells, due to their unique differentiation and CO2 storage role, are identified as valuable subjects for large-scale cellular signature profiling.

Conclusions:

  • The developed pipelines provide a powerful approach for dissecting cellular signatures in a tissue-specific manner.
  • These methods are versatile and applicable to a wide range of biological samples.
  • Xylem cells present a unique and relevant system for advanced cellular profiling studies.