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Updated: Jul 12, 2025
In Vitro Model of Coronary Angiogenesis
Published on: March 10, 2020
A sandwiched ventricular explant assay to model mouse coronary angiogenesis ex vivo
Pengfei Lu1, Bingruo Wu2, Yidong Wang3
1Precision Research Center for Refractory Diseases, Institute for Clinical Research, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China; Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Abstract:
Developing an ex vivo system that mimics in vivo developmental coronary angiogenesis provides an improved understanding of its underlying molecular and cellular mechanisms. Here, we present a sandwiched embryonic ventricular explant assay to model mouse coronary angiogenesis ex vivo. We describe steps for breeding mice, labeling endocardial cells, isolating murine hearts, dissecting left ventricles, and making sandwiched explants in Matrigel. We then detail procedures for modeling coronary angiogenesis and taking images. For complete details on the use and execution of this protocol, please refer to Lu et al. (2023)1.
