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Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Related Experiment Video

Updated: Jul 12, 2025

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

Protein-coding potential of non-canonical open reading frames in human transcriptome.

Hitesh Kore¹, Keshava K Datta², Shivashankar H Nagaraj³

¹Centre for Genomics and Personalised Health, Queensland University of Technology, Brisbane, Queensland, 4059, Australia; Cancer Precision Medicine Group, QIMR Berghofer Medical Research Institute, 300 Herston Road, Herston, Queensland, 4006, Australia; Faculty of Health, Queensland University of Technology, Brisbane, Queensland, 4059, Australia.

Biochemical and Biophysical Research Communications

|October 28, 2023

Summary

Recent studies reveal numerous small open reading frame encoded proteins (SEPs) from human noncoding DNA. While many are poorly conserved, some SEPs are crucial for biological functions and diseases.

Keywords:

Non-coding RNAs Novel proteins Protein-coding potential SEPs

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Published on: April 11, 2019

An Integrated Approach for Microprotein Identification and Sequence Analysis

An Integrated Approach for Microprotein Identification and Sequence Analysis

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Last Updated: Jul 12, 2025

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

An Integrated Approach for Microprotein Identification and Sequence Analysis

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

Area of Science:

Genomics
Proteomics
Molecular Biology

Background:

Proteogenomics and ribosome profiling reveal novel proteins from human noncoding genome regions.
These small open reading frame encoded proteins (SEPs) originate from untranslated regions (UTRs) of mRNAs and long non-coding RNAs (lncRNAs).
SEPs are typically short (<150 amino acids) and exhibit limited evolutionary conservation.

Conclusions:

The full extent and functional importance of novel protein-coding regions in the human genome remain incompletely understood.
Continued research is vital for accurate annotation and understanding the roles of SEPs.
SEPs represent a significant, yet underexplored, component of the human proteome.