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Related Experiment Video

Updated: Jul 12, 2025

Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation
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Conductive electrospun polymer improves stem cell-derived cardiomyocyte function and maturation.

Gisselle Gonzalez1, Aileena C Nelson1, Alyssa R Holman2

  • 1Shu Chien-Gene Lay Department of Bioengineering, La Jolla, CA, 92093, USA.

Biomaterials
|October 28, 2023
PubMed
Summary
This summary is machine-generated.

Conductive polymer scaffolds improve the maturation and electrical communication of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs). These stable scaffolds enhance cardiac gene expression and function, aiding cardiac disease modeling and therapy development.

Keywords:
Calcium handlingDesmoplakinFluoVoltSarcomere organizationpoly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS)poly(vinyl) alcohol (PVA)

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Area of Science:

  • Biomedical Engineering
  • Stem Cell Biology
  • Cardiovascular Research

Background:

  • Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) often exhibit immaturity and poor electrical coupling.
  • Developing functional hPSC-CMs is crucial for disease modeling and regenerative medicine.

Purpose of the Study:

  • To investigate the use of conductive polymer fiber meshes to enhance electrical communication and maturation of hPSC-CMs.
  • To assess the stability and biocompatibility of conductive scaffolds for cardiac cell culture.

Main Methods:

  • Electrospinning of conductive polymers to create fiber mesh scaffolds.
  • Seeding hPSC-CMs on conductive and non-conductive scaffolds.
  • Characterization of scaffold properties (stability, stiffness, conductivity).
  • Assessment of cell viability, adherence, gene expression, structural protein organization, and calcium handling.

Main Results:

  • Conductive scaffolds were stable, matched in vivo cardiac stiffness, and exhibited tunable electrical conductivity.
  • hPSC-CMs remained viable and adhered to scaffolds for at least 5 days.
  • Conductive substrates upregulated cardiac/muscle genes, improved structural protein organization, enhanced calcium handling, and promoted membrane depolarization compared to non-conductive controls, even at sub-confluent densities.

Conclusions:

  • Blended conductive scaffolds are stable and support electrical coupling in hPSC-CMs.
  • These scaffolds promote hPSC-CM maturation, improving electrical properties and cardiac gene expression.
  • Conductive scaffolds offer a promising strategy for advancing cardiac disease modeling and therapeutic development using hPSC-CMs.