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Acoustic droplet ejection facilitates cell-based high-throughput screenings using natural products
Thomas A Mackenzie1, José R Tormo1, Bastien Cautain1
1Fundación MEDINA, Av. Conocimiento 34, Parque Tecnológico de la Salud, 18016 Granada, Spain.
SLAS Technology
|October 28, 2023
Summary
Acoustic Droplet Ejection (ADE) effectively dispenses natural product (NP) extracts for cell-based high-throughput screening (HTS). This technology addresses challenges with small volumes and varying viscosity, advancing drug discovery efforts.
Area of Science:
- Drug Discovery and Development
- Natural Product Chemistry
- Biotechnology and Bioengineering
Background:
- Natural Products (NPs) are a primary source for drug discovery, with many approved drugs originating from or inspired by NPs.
- High-Throughput Screening (HTS) is crucial for identifying new drug candidates from NP libraries.
- Challenges in industrial-scale HTS of NP libraries include handling small extract volumes and viscosity variations.
Purpose of the Study:
- To evaluate the efficacy of Acoustic Droplet Ejection (ADE) for dispensing natural product extract libraries.
- To assess the suitability of ADE technology for cell-based high-throughput screening (HTS) assays.
Main Methods:
- Investigated the use of Acoustic Droplet Ejection (ADE) for precise liquid handling of natural product extracts.
- Applied ADE technology in cell-based high-throughput screening (HTS) assays to test its performance.
Main Results:
- Demonstrated the effectiveness of ADE in accurately dispensing variable volumes of natural product extracts.
- Confirmed the successful application of ADE for NP library screening in cell-based assays, overcoming previous limitations.
Conclusions:
- Acoustic Droplet Ejection (ADE) is a viable and efficient technology for miniaturizing reagent volumes in natural product high-throughput screening.
- ADE facilitates the industrial-scale screening of natural product libraries, supporting the discovery of new chemical entities for drug development.
