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Apoptosis-inducing, anti-angiogenic and anti-migratory effects of a dinuclear Pd(II) complex on breast cancer: A

Merve Erkisa Genel1, Kaan Adacan2, Selin Selvi3

  • 1Istinye University, Molecular Cancer Research Center (ISUMKAM), Istanbul, Turkey; Istanbul Health and Technology University, Faculty of Medicine, Department of Medical Biology, Istanbul, Turkey.

Microvascular Research
|October 28, 2023
PubMed

Insights

A novel dinuclear palladium(II) complex shows significant anti-cancer effects against breast cancer cells. This compound induces apoptosis, inhibits metastasis, and suppresses angiogenesis, suggesting its potential for breast cancer treatment.

Area of Science:

  • Inorganic Chemistry
  • Medicinal Chemistry
  • Cancer Biology

Background:

  • Breast cancer management necessitates novel therapeutic compounds due to high mortality and morbidity.
  • Multinuclear metal complexes are being explored for their DNA binding affinity and cytotoxic potential.

Purpose of the Study:

  • To investigate the anti-growth and cytotoxic effects of a dinuclear Palladium(II) complex on human breast cancer cell lines (MCF-7 and MDA-MB-231).
  • To evaluate the complex's impact on cancer cell metastasis and angiogenesis.

Main Methods:

  • Cell viability assays, staining methods, flow cytometry, and immunoblotting were used to assess cytotoxicity and apoptosis.
  • Colony formation, invasion, and migration assays were performed to evaluate anti-metastatic properties.
  • The Chorioallantoic Membrane (CAM) assay was employed to assess anti-angiogenic effects.

Main Results:

  • The dinuclear Pd(II) complex induced apoptosis in both MCF-7 and MDA-MB-231 cells, evidenced by increased caspase-3/7 levels and positive annexin V staining.
  • Apoptosis was confirmed through altered TNFR1 and TRADD expression, caspase-8 cleavage, BCL-2 inactivation, and loss of mitochondrial membrane potential.
  • The complex demonstrated anti-migration properties and significantly inhibited angiogenesis in the CAM assay.

Conclusions:

  • The dinuclear Pd(II) complex exhibits potent anti-cancer properties, including induction of apoptosis, inhibition of metastasis, and anti-angiogenesis.
  • These findings highlight the therapeutic potential of this dinuclear Pd(II) complex for breast cancer treatment.
  • Further in vivo studies are warranted to validate its efficacy and safety for clinical application.
Keywords:
ApoptosisBreast cancerCAM assayInvasionPalladium

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