Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

CD4<sup>+</sup> T cells impair tumor growth through IL-3 and TNF-dependent vascular damage.

Science (New York, N.Y.)·2026

Same author

High-Plex Tissue Imaging with Conventional Immunofluorescence Platforms and Open-Source Software via Iterative Bleaching Extends Multiplexity (IBEX).

Methods in molecular biology (Clifton, N.J.)·2026

Same author

Agonistic anti-CD40 antibody treatment converts resident regulatory T cells into activated type 1 effectors within the tumor microenvironment.

Immunity·2026

Same author

Immunological knowledge.

Nature immunology·2026

Same author

Tumour-brain crosstalk restrains cancer immunity via a sensory-sympathetic axis.

Nature·2026

Same author

The IBEX knowledge-base a community resource enabling adoption and development of immunofluorescence imaging methods.

eLife·2026

Same journal

Systemic immune profiling in hepatocellular carcinoma: navigating etiological heterogeneity and selection bias.

Journal for immunotherapy of cancer·2026

Same journal

Long-term follow-up from the OMNIVORE trial: response-adaptive nivolumab and ipilimumab in advanced renal cell carcinoma.

Journal for immunotherapy of cancer·2026

Same journal

PARP inhibition combined with a T-cell receptor β chain-directed antibody fusion molecule drives polyclonal antitumor immunity and tumor regression.

Journal for immunotherapy of cancer·2026

Same journal

ENPP3 CAR T cells combined with CD206 modulation suppress adrenocortical carcinoma.

Journal for immunotherapy of cancer·2026

Same journal

Metabolic inhibition of glutamate-cysteine ligase increases dendritic cell-mediated antitumor immunity in melanoma.

Journal for immunotherapy of cancer·2026

Same journal

Correction: CXCR6 is required for antitumor efficacy of intratumoral CD8+ T cell.

Journal for immunotherapy of cancer·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 22, 2026

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Published on: January 21, 2019

Multiplex imaging in immuno-oncology.

Chen Zhao^1,2, Ronald N Germain³

¹Thoracic and GI Malignancies Branch, CCR, NCI, Bethesda, Maryland, USA.

Journal for Immunotherapy of Cancer

|October 29, 2023

Summary

This summary is machine-generated.

Multiplex imaging is a powerful tool for studying the tumor microenvironment (TME) in immuno-oncology. This review guides researchers through multiplex imaging techniques and analysis for discovering biomarkers and therapeutic targets.

Keywords:

immunotherapy tumor microenvironment

More Related Videos

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Author Spotlight: Unlocking Insights into the Immune Cell Landscape of Tumors

Author Spotlight: Unlocking Insights into the Immune Cell Landscape of Tumors

Published on: August 18, 2023

Related Experiment Videos

Last Updated: Jun 22, 2026

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Published on: January 21, 2019

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Author Spotlight: Unlocking Insights into the Immune Cell Landscape of Tumors

Author Spotlight: Unlocking Insights into the Immune Cell Landscape of Tumors

Published on: August 18, 2023

Area of Science:

Immunology
Oncology
Biotechnology

Background:

Multiplex imaging allows detailed examination of the tumor microenvironment (TME).
It reveals interactions between immune cells, stroma, matrix, and malignant cells.
This technology is crucial for advancing immuno-oncology research.

Purpose of the Study:

To provide an overview of multiplex imaging in immuno-oncology.
To guide novice researchers on fundamental principles of multiplex imaging and analysis.
To highlight the potential of multiplex imaging for biomarker discovery and therapeutic target identification.

Main Methods:

Review of current multiplex imaging techniques.
Discussion of study design considerations.
Outline of experiment optimization strategies.
Overview of data analysis approaches.

Main Results:

Multiplex imaging offers deep insights into the TME.
Challenges in study design, optimization, and analysis were identified.
The potential for discovering novel biomarkers and therapeutic targets is significant.

Conclusions:

Multiplex imaging is essential for modern immuno-oncology.
Addressing current challenges will enhance its utility.
This review serves as a foundational guide for researchers.