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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Efficient Large DNA Fragment Knock-in by Long dsDNA with 3'-Overhangs Mediated CRISPR Knock-in (LOCK) in Mammalian

Wenjie Han1, Haojun Liang1, Jianqiang Bao2,3

  • 1Hefei National Research Center for Physical Sciences at the Microscale, School of Chemistry and Materials Science, Department of Polymer Science and Engineering, Chinese Academy of Sciences Key Laboratory of Soft Matter Chemistry, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei, Anhui, China.

Bio-Protocol
|October 30, 2023
PubMed

Keywords:
CRISPR/Cas9Cas9-PCV2 fusionGene knock-in (KI)Genome editingHomologous recombinationdsDNA donorodsDNA donorssDNA donor

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