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Malate-Based Biodegradable Scaffolds Activate Cellular Energetic Metabolism for Accelerated Wound Healing.
Min Wu1, Yitao Zhao1, Meihan Tao1
1Department of Histology and Embryology, GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, NMPA Key Laboratory for Safety Evaluation of Cosmetics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, P. R. China.
ACS Applied Materials & Interfaces
|October 30, 2023
Summary
This study developed novel poly(diol l-malate) scaffolds that enhance cellular energy production. These bioenergetic scaffolds promote tissue regeneration and accelerate wound healing by boosting cellular metabolism.
Area of Science:
- Biomaterials Science
- Cellular Bioenergetics
- Tissue Engineering
Background:
- Cellular bioenergetics offers therapeutic potential for tissue regeneration.
- Developing 3D scaffolds with long-term bioenergetic effects for tissue repair is challenging.
Purpose of the Study:
- To investigate the potential of l-malate in promoting cellular energy metabolism.
- To fabricate and characterize novel bioenergetic porous scaffolds for tissue regeneration.
Main Methods:
- Synthesized poly(diol l-malate) (PDoM) prepolymers via one-pot polycondensation.
- Fabricated porous PDoM scaffolds through thermal cross-linking.
- Evaluated scaffold degradation products' effect on cellular metabolism and ATP levels.
- Assessed scaffold's ability to support mesenchymal stem cell (MSC) growth and bioactive molecule secretion.
- Tested scaffold efficacy in a rat full-thickness skin defect model.
Main Results:
- PDoM scaffolds' degradation products enhance cellular energy metabolism by participating in the tricarboxylic acid (TCA) cycle, increasing adenosine triphosphate (ATP) levels.
- Scaffolds promoted cellular biosynthesis, including collagen type I, fibronectin 1, and actin alpha 2.
- PDoM scaffolds supported MSC growth and stimulated secretion of vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1), and basic fibroblast growth factor (bFGF).
- Stem cell-laden scaffolds significantly accelerated wound healing in a rat model.
Conclusions:
- Developed PDoM scaffolds effectively promote cellular bioenergetics and tissue regeneration.
- The scaffolds provide a promising platform for therapeutic applications in regenerative medicine.
- PDoM scaffolds represent a significant advancement in creating functional biomaterials for wound healing.
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