Related Experiment Video
Updated: Jul 12, 2025
09:04
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
Published on: September 25, 2019
8.3K
Recent Research Trends in Stem Cells Using CRISPR/Cas-Based Genome Editing Methods
Da Eun Yoon1,2, Hyunji Lee1,3, Kyoungmi Kim1,2
1Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Korea.
International Journal of Stem Cells
|October 31, 2023
Summary
CRISPR gene editing technology enables precise DNA alterations in organisms, including stem cells. This review explores CRISPR/Cas applications in stem cell research for disease understanding and novel therapeutic strategies.
Area of Science:
- Genomics
- Molecular Biology
- Stem Cell Research
Background:
- The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system is a powerful genome editing technology.
- CRISPR allows for precise DNA modifications, including single nucleotide conversions, gene knock-ins, chromosomal rearrangements, and gene disruptions.
- CRISPR-based epigenetic regulation can be achieved without inducing DNA damage.
Purpose of the Study:
- To review the latest advancements in stem cell research utilizing CRISPR/Cas technologies.
- To discuss the potential of CRISPR-mediated stem cell engineering in understanding disease pathogenesis.
- To explore future prospects of CRISPR applications in treating incurable diseases.
Main Methods:
- Review of current CRISPR/Cas technologies applied to stem cell research.
- Analysis of gene editing capabilities for precise DNA alterations.
- Examination of epigenetic regulation strategies using CRISPR systems.
Main Results:
- CRISPR technology facilitates diverse and precise genome editing in stem cells.
- CRISPR applications offer insights into disease mechanisms and potential therapeutic targets.
- CRISPR-Cas systems are being explored for epigenetic modifications in stem cells.
Conclusions:
- CRISPR/Cas technologies hold significant promise for stem cell engineering.
- Stem cell gene editing is crucial for advancing disease research and developing treatments.
- Future applications of CRISPR in stem cell therapy are extensive for various diseases.
Related Concept Videos
01:59CRISPR
52.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.0K
02:53CRISPR and crRNAs
17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
01:28CRISPR/Cas9 Genome Editing
23
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
23
02:31Homologous Recombination
50.6K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.6K