Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dual responsive enzyme mimicking activity of AgX (X=Cl, Br, I) nanoparticles and its application for cancer cell detection.

ACS applied materials & interfaces·2014

Same author

Naphthoquinone-directed C-H annulation and C(sp³)-H bond cleavage: one-pot synthesis of tetracyclic naphthoxazoles.

The Journal of organic chemistry·2014

Same author

Pulmonary toxicity in mice following exposure to cerium chloride.

Biological trace element research·2014

Same author

Role of surgery in the treatment of patients with high-risk neuroblastoma who have a poor response to induction chemotherapy.

Journal of pediatric surgery·2014

Same author

Glutathione-S-transferase polymorphisms (GSTM1, GSTT1 and GSTP1) and acute leukemia risk in Asians: a meta-analysis.

Asian Pacific journal of cancer prevention : APJCP·2014

Same author

Influence of casting solvent on phenyl ordering at the surface of spin cast polymer thin films.

Journal of colloid and interface science·2014

Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026

Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026

Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026

Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026

Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026

Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 12, 2025

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells

Published on: November 10, 2023

High-throughput single-cell sorting by stimulated Raman-activated cell ejection.

Jing Zhang^1,2, Haonan Lin^1,2, Jiabao Xu³

¹Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.

Biorxiv : the Preprint Server for Biology

|October 31, 2023

Summary

This summary is machine-generated.

A new stimulated Raman-activated cell ejection (S-RACE) platform achieves high-throughput single-cell sorting using chemical imaging. This method enables precise cell isolation for applications in biology and medicine.

Keywords:

Biological Sciences Biophysics and Computational Biology Raman spectroscopy cell ejection cell sorting high-throughput analysis laser-induced forward transfer single microorganism isolation and culture

More Related Videos

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Related Experiment Videos

Last Updated: Jul 12, 2025

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells

Published on: November 10, 2023

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Area of Science:

Biotechnology
Chemical Imaging
Cell Biology

Background:

Single-cell sorting is crucial for understanding cellular heterogeneity.
Raman-activated cell sorting (RACS) offers label-free sorting but faces throughput limitations.
Spontaneous Raman scattering has a small cross-section, limiting current RACS speed.

Conclusions:

The S-RACE platform provides high-throughput, label-free single-cell sorting.
Enables live cell sorting for subsequent culture of bacteria and fungi.
Opens new avenues for single-cell applications in diverse biological and medical fields.