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Updated: Jul 12, 2025
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High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells
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Physiological characterization of single gene lysis proteins
S Francesca Antillon1,2, Thomas G Bernhardt3,4, Karthik Chamakura1,2
1Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843-2128, United States.
Biorxiv : the Preprint Server for Biology
|October 31, 2023
Summary
New methods distinguish single gene lysis proteins (Sgls) into type I and type II. This classification helps identify novel cell envelope targets for developing new protein antibiotic therapies.
Area of Science:
- Microbiology
- Molecular Biology
- Bacteriology
Background:
- Eleven single gene lysis proteins (Sgls) are known, with three (Qβ A2, ϕX174 E, Lys) inhibiting peptidoglycan (PG) biosynthesis.
- These Sgls are termed 'protein antibiotics' due to their cell lysis mechanism.
- Thousands of new Sgl genes are predicted from recent metagenomic data, necessitating classification methods.
Conclusions:
- New methods allow rapid classification of novel Sgls.
- Structural similarity is not a reliable indicator for Sgl classification.
- This work facilitates the discovery of new Sgls and potential antibiotic targets.
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