Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

1.1K
The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
1.1K
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

1.1K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
1.1K
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

704
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
704
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

814
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
814
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

868
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
868
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.1K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PANoptosis in diabetic retinopathy: immunological insights into mechanisms and translational therapies.

Frontiers in immunology·2026
Same author

The G protein-casein kinase 2 module acts as a pivot in plasma membrane-to-nucleus CLAVATA signaling to control shoot apical meristem size.

Nature communications·2026
Same author

Extended difficulties after psychedelic experiences: Prevalence and associations in a global, multilingual sample.

Research square·2026
Same author

The DREB2C.L-IAGLU module contributes to long-term heat stress via sugar metabolism in cucumber.

Horticulture research·2026
Same author

The Microtubule-Associated Protein CsTON2 Interacts With CsTRM5 and CsSUN to Regulate Fruit Shape Development in Cucumber.

Plant biotechnology journal·2025
Same author

Towards Closed-Loop Neuromodulation for Type 2 Diabetes With Ex Vivo Validation of Beta-Cell Activity and FOPP Detection.

IEEE transactions on biomedical circuits and systems·2025

Related Experiment Video

Updated: Jul 12, 2025

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

5.2K

A portable NMR platform with arbitrary phase control and temperature compensation.

Qing Yang1, Jianyu Zhao1, Frederik Dreyer1

  • 1Institute of Smart Sensors, University of Stuttgart, Pfaffenwaldring 47, 70569 Stuttgart, Germany.

Magnetic Resonance (Gottingen, Germany)
|October 31, 2023
PubMed
Summary

This study introduces a novel nuclear magnetic resonance (NMR) platform using a CMOS NMR-on-a-chip transceiver. The system offers precise phase control and digital temperature compensation for advanced NMR measurements.

More Related Videos

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

5.6K
Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.2K

Related Experiment Videos

Last Updated: Jul 12, 2025

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

5.2K
High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

5.6K
Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.2K

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Electrical Engineering

Background:

  • Traditional Nuclear Magnetic Resonance (NMR) systems often require complex hardware for precise signal generation and temperature stabilization.
  • Integration of NMR components onto a single chip (NMR-on-a-chip) offers potential for miniaturization and cost reduction.
  • Achieving arbitrary phase control and phase-coherent detection is crucial for advanced NMR techniques like spectroscopy and relaxometry.

Purpose of the Study:

  • To present a custom-designed NMR platform integrating a CMOS NMR-on-a-chip transceiver and a synchronous reference signal generator.
  • To demonstrate arbitrary phase control of excitation pulses and phase-coherent detection at a non-zero intermediate frequency (IF).
  • To introduce a digital temperature compensation scheme using a direct digital synthesis (DDS)-based frequency generator, eliminating the need for extra hardware.

Main Methods:

  • Development of a broadband complementary metal-oxide-semiconductor (CMOS) NMR-on-a-chip transceiver.
  • Implementation of a synchronous reference signal generator with arbitrary phase control capabilities.
  • Integration of a direct digital synthesis (DDS)-based frequency generator for digital temperature compensation.
  • Verification of system functionality through NMR spectroscopy and relaxometry measurements.

Main Results:

  • Successful demonstration of arbitrary phase control for NMR excitation pulses.
  • Validation of phase-coherent detection at a non-zero intermediate frequency (IF).
  • Confirmation of the effectiveness of the digital temperature compensation scheme, mimicking field locking without additional hardware.
  • Overall state-of-the-art performance of the integrated NMR system.

Conclusions:

  • The presented custom-designed NMR platform, featuring a CMOS NMR-on-a-chip transceiver and DDS-based frequency generator, offers advanced capabilities.
  • The system successfully integrates precise phase control and digital temperature compensation, enhancing NMR measurement precision and stability.
  • The validated performance indicates significant advancements in NMR hardware design and potential for broader applications.