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Related Experiment Videos

A high potential acceptor for photosystem II.

J M Bowes, A R Crofts, S Itoh

    Biochimica Et Biophysica Acta
    |August 14, 1979
    PubMed
    Summary
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    Researchers investigated ferricyanide

    Area of Science:

    • Plant Physiology
    • Photosynthesis Research
    • Biochemistry

    Background:

    • Photosystem II (PSII) is crucial for light-dependent reactions in photosynthesis.
    • Understanding electron transport in PSII is key to comprehending energy conversion.
    • Ferricyanide is a common oxidant used to probe electron transfer in PSII.

    Purpose of the Study:

    • To investigate the effects of ferricyanide on Photosystem II reactions.
    • To identify and characterize a potential secondary electron acceptor (Q2) in PSII.
    • To determine the redox potential and kinetics of this secondary acceptor.

    Main Methods:

    • Measurements of microsecond and millisecond prompt fluorescence.
    • Measurements of microsecond-delayed fluorescence in dark-adapted chloroplasts.

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  • Titrations using ferri-ferrocyanide mixtures and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) inhibition.

    • Main Results:

    • A pH-dependent midpoint potential of 400 mV at pH 7.0 was determined for a secondary acceptor (Q2).
    • Ferricyanide pre-incubation and DCMU inhibition altered fluorescence induction kinetics and delayed fluorescence.
    • Evidence suggests Q2 is located before the DCMU binding site and reduces within 5 µs.

    Conclusions:

    • The study suggests the presence of a secondary electron acceptor, Q2, in Photosystem II.
    • Q2 has a redox potential of Em7 = 400 mV and is present at 0.35–1 equivalent per reaction center.
    • Q2 plays a role in the double turnover of photochemistry within a single flash.