Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Microbial metabolism in deep terrestrial subsurface communities - amino acids as biosignatures.

Current research in microbial sciences·2026

Same author

Seasonal variation in metabolic profiles and microbial communities in a subarctic ore processing plant.

Environmental microbiology reports·2024

Same author

Altering environmental conditions induce shifts in simulated deep terrestrial subsurface bacterial communities-Secretion of primary and secondary metabolites.

Environmental microbiology·2023

Same author

Corrigendum to "Anionic nanocellulose as competing agent in microbial DNA extraction from mine process samples" [Journal of Microbiological Methods volume 215 (2023) 106850].

Journal of microbiological methods·2023

Same author

Editorial: Geomicrobes: Life in terrestrial deep subsurface, volume II.

Frontiers in microbiology·2023

Same author

Implications of a short carbon pulse on biofilm formation on mica schist in microcosms with deep crystalline bedrock groundwater.

Frontiers in microbiology·2023

Search research articles

Related Experiment Video

Updated: Jul 12, 2025

Fabrication of a Functionalized Magnetic Bacterial Nanocellulose with Iron Oxide Nanoparticles

Fabrication of a Functionalized Magnetic Bacterial Nanocellulose with Iron Oxide Nanoparticles

Published on: May 26, 2016

Anionic nanocellulose as competing agent in microbial DNA extraction from mine process samples.

Malin Bomberg¹, Hanna Miettinen¹

¹VTT Technical Research Centre of Finland, P.O. Box 1000, 02044 VTT, Finland.

Journal of Microbiological Methods

|October 31, 2023

Summary

A new anionic nanocellulose method effectively detaches microorganisms and their DNA from mineral particles in flotation and minerals processing. This approach significantly improves DNA yield and microbial community detection compared to commercial kits.

Keywords:

16S rRNA gene sequencing Microbe-mineral interaction Mineral flotation Molecular techniques Nucleic acid adsorption fungi

More Related Videos

Extraction of High Molecular Weight DNA from Microbial Mats

Extraction of High Molecular Weight DNA from Microbial Mats

Published on: July 7, 2011

Highly Stable, Functional Hairy Nanoparticles and Biopolymers from Wood Fibers: Towards Sustainable Nanotechnology

Highly Stable, Functional Hairy Nanoparticles and Biopolymers from Wood Fibers: Towards Sustainable Nanotechnology

Published on: July 20, 2016

Related Experiment Videos

Last Updated: Jul 12, 2025

Fabrication of a Functionalized Magnetic Bacterial Nanocellulose with Iron Oxide Nanoparticles

Fabrication of a Functionalized Magnetic Bacterial Nanocellulose with Iron Oxide Nanoparticles

Published on: May 26, 2016

Extraction of High Molecular Weight DNA from Microbial Mats

Extraction of High Molecular Weight DNA from Microbial Mats

Published on: July 7, 2011

Highly Stable, Functional Hairy Nanoparticles and Biopolymers from Wood Fibers: Towards Sustainable Nanotechnology

Highly Stable, Functional Hairy Nanoparticles and Biopolymers from Wood Fibers: Towards Sustainable Nanotechnology

Published on: July 20, 2016

Area of Science:

Environmental Science
Microbiology
Materials Science

Background:

Microorganisms impact metal concentrate grade and yield in flotation and minerals processing.
Strong adherence of microbes and their DNA to mineral particles complicates microbial studies.
Effective techniques are needed to detach these microorganisms and their genetic material from mineral surfaces.

Purpose of the Study:

To develop a novel method for detaching microorganisms and their DNA from mineral particles.
To improve the extraction efficiency of microbial DNA from ore processing samples.
To provide a more comprehensive understanding of microbial communities in mineral processing environments.

Main Methods:

Utilized anionic nanocellulose as a competitive agent to block DNA sorption and detach microbial cells.
Compared DNA yield and microbial gene copy numbers obtained by the custom nanocellulose method (CM) against a commercial kit (KIT).
Analyzed microbial community structure using quantitative PCR (qPCR) on extracted DNA.

Main Results:

The custom nanocellulose method (CM) yielded up to one ng DNA mL^-1, significantly higher than commercial kits (KIT) which were below detection limits.
CM detected 0.5-4 orders of magnitude more bacterial 16S and fungal 5.8S rRNA gene copies than KIT.
Distinct differences in microbial community structure were observed between CM and KIT extracted samples.

Conclusions:

Anionic nanocellulose effectively reduces microbial and DNA-mineral interactions, enhancing microbial DNA extraction.
The custom nanocellulose method provides a more comprehensive view of microbial communities in mineral processing than standard commercial kits.
This method offers improved insights into microbial roles in ore processing, potentially optimizing metal recovery.