Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 12, 2025

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae
05:39

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

Published on: December 2, 2022

2.5K

A rapid PCR-based method to determine the Neurospora crassa mating type.

Anke Schäfer1, Frank Kempken1

  • 1Abteilung Botanische Genetik und Molekularbiologie, Botanisches Institut und Botanischer Garten, Kiel, Germany.

Journal of Basic Microbiology
|October 31, 2023
PubMed
Summary

Determining mating types in Neurospora crassa previously required difficult test crosses. A new, rapid polymerase chain reaction (PCR)-based method now simplifies mating type determination in N. crassa.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Iron allocation to chloroplast proteins depends on the DNA-binding protein WHIRLY1.

Planta·2025
Same author

Over-accumulation of chloroplast-nucleus located WHIRLY1 in barley leads to a decrease in growth and an enhanced stress resistance.

The Plant journal : for cell and molecular biology·2024
Same author

Marine fungi: A treasure trove of novel natural products and for biological discovery.

PLoS pathogens·2023
Same author

Insights into Taxol® biosynthesis by endophytic fungi.

Applied microbiology and biotechnology·2023
Same author

Transcriptomic analysis of poco1, a mitochondrial pentatricopeptide repeat protein mutant in Arabidopsis thaliana.

BMC plant biology·2020
Same author

PRECOCIOUS1 (POCO1), a mitochondrial pentatricopeptide repeat protein affects flowering time in Arabidopsis thaliana.

The Plant journal : for cell and molecular biology·2019

Area of Science:

  • Mycology
  • Molecular Biology
  • Genetics

Background:

  • Mating type determination is crucial for fungal reproduction and genetic studies.
  • Current methods for Neurospora crassa mating type determination rely on laborious test crosses with known strains.

Purpose of the Study:

  • To develop a straightforward and efficient method for identifying mating types in Neurospora crassa.
  • To replace traditional, time-consuming cross-based methods with a molecular approach.

Main Methods:

  • Development of a polymerase chain reaction (PCR)-based assay.
  • Utilizing specific primers to amplify mating type-specific DNA sequences in Neurospora crassa.

Main Results:

  • The PCR-based method provides accurate mating type determination.
Keywords:
Neurospora crassaPCR-based mating type identificationmating type

More Related Videos

Sexual Crosses with the Mucoromycete Phycomyces blakesleeanus
05:34

Sexual Crosses with the Mucoromycete Phycomyces blakesleeanus

Published on: June 6, 2025

115
Microscopy of Fission Yeast Sexual Lifecycle
07:47

Microscopy of Fission Yeast Sexual Lifecycle

Published on: March 9, 2016

14.6K

Related Experiment Videos

Last Updated: Jul 12, 2025

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae
05:39

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

Published on: December 2, 2022

2.5K
Sexual Crosses with the Mucoromycete Phycomyces blakesleeanus
05:34

Sexual Crosses with the Mucoromycete Phycomyces blakesleeanus

Published on: June 6, 2025

115
Microscopy of Fission Yeast Sexual Lifecycle
07:47

Microscopy of Fission Yeast Sexual Lifecycle

Published on: March 9, 2016

14.6K
  • This technique is significantly faster and more reliable than conventional methods.

    • Conclusions:

    • A simple, quick, and reliable PCR-based method for mating type determination in Neurospora crassa has been established.
    • This molecular tool streamlines genetic research and strain characterization in N. crassa.