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The DNA Replication Fork01:02

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
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The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
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Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
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A DNA Replication Stress-Based Prognostic Model for Lung Adenocarcinoma.

S Shi1, G Wen1, C Lei1

  • 1Department of Cardiothoracic Surgery, The People's Hospital of Dazu District, Chongqing, 402360 China.

Acta Naturae
|November 1, 2023
PubMed
Summary

This study developed a 10-gene model predicting lung adenocarcinoma (LUAD) prognosis based on DNA replication stress. The model identifies high-risk patients with poorer survival and potential immunotherapy benefits for low-risk patients.

Keywords:
DNA replication stressanti- tumor drug predictionimmunotherapy responselung adenocarcinomaprognostic model

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Tumor cells experience constant DNA replication stress, a factor in cancer development.
  • The prognostic significance of DNA replication stress in lung adenocarcinoma (LUAD) remains under-explored.

Purpose of the Study:

  • To investigate the prognostic value of DNA replication stress-related genes (DNARSs) in LUAD.
  • To develop and validate a predictive model for LUAD patient prognosis.

Main Methods:

  • Identified differentially expressed genes (DEGs) related to DNARSs in the TCGA-LUAD dataset.
  • Constructed a 10-gene prognostic model using Cox regression analysis.
  • Validated the model using ROC curves, Kaplan-Meier survival analysis, and nomograms incorporating clinical data.

Main Results:

  • The 10-gene model effectively predicted LUAD prognosis, with a high-risk group showing poorer survival.
  • The Riskscore derived from the model was an independent prognostic predictor.
  • Low-risk patients demonstrated increased immune cell infiltration and function, suggesting better immunotherapy response.

Conclusions:

  • A novel 10-gene prognostic model for LUAD based on DNA replication stress was successfully developed and validated.
  • The model aids in prognostic evaluation and can inform treatment strategies, predicting immunotherapy response and chemotherapy sensitivity.