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[Evaluation of the lower urinary tract function using voiding spot assay in mice]
Chuang Luo1, Jia-Li Yang1, Juan Liu1
1School of Basic Medical Sciences, Southwest Medical University, Luzhou 646000, China.
Sheng Li Xue Bao : [Acta Physiologica Sinica]
|November 1, 2023
Summary
A new voiding spot assay (VSA) system standardizes lower urinary tract function evaluation in mice. This reliable method uses UV light and filter paper to analyze urine output and patterns in healthy and stressed mice.
Area of Science:
- Urology
- Animal Models
- Biomedical Assays
Context:
- Evaluating lower urinary tract function in mice is crucial for preclinical research.
- Existing methods may lack standardization or simplicity for widespread use.
- Standardized protocols are needed for reproducible animal model studies.
Purpose:
- To develop and standardize a simple, reliable voiding spot assay (VSA) system for assessing mouse lower urinary tract function.
- To optimize filter paper selection and UV light parameters for accurate urine spot analysis.
- To validate the VSA system across different laboratories and in disease models.
Summary:
- A voiding spot assay (VSA) system was developed using No. 4 filter paper and 365 nm UV light.
- A linear correlation was established between urine volume and stained area, enabling quantitative analysis.
- The system accurately measured urine output in wild-type mice and detected alterations in urinary tract infection and stress models.
Impact:
- Establishes a standardized protocol for evaluating mouse lower urinary tract function.
- Provides a reliable tool for preclinical research on urological conditions and stress responses.
- Facilitates cross-laboratory comparisons and enhances the reproducibility of mouse voiding studies.
