Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Energetics of Solution Formation02:35

Energetics of Solution Formation

6.8K
The formation of a solution is an example of a spontaneous process, which is a process that occurs under specified conditions without energy from some external source.
When the strengths of the intermolecular forces of attraction between solute and solvent species in a solution are no different than those present in the separated components, the solution is formed with no accompanying energy change. Formation of the solution requires the solute–solute and solvent–solvent...
6.8K
Solubility Equilibria03:07

Solubility Equilibria

52.7K
Solubility equilibria are established when the dissolution and precipitation of a solute species occur at equal rates. These equilibria underlie many natural and technological processes, ranging from tooth decay to water purification. An understanding of the factors affecting compound solubility is, therefore, essential to the effective management of these processes. This section applies previously introduced equilibrium concepts and tools to systems involving dissolution and precipitation.
The...
52.7K
Solubility Equilibria: Overview01:09

Solubility Equilibria: Overview

691
When a substance such as sodium chloride is added to water, it dissolves, forming an aqueous solution. The extent of dissolution is called solubility. The process of dissolution can exist in equilibrium, just like other chemical processes. Solubility equilibria are also called precipitation equilibria because the process of solubility can be reversible. The reverse of the solubility process is called precipitation.
Solubility is important in biological and environmental processes. A notable...
691

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular Simulation of Hydrogen Systems: From Properties and Methods to Applications and Future Directions.

Chemical reviews·2025
Same author

Tailoring precipitates for enhanced hydrogen trapping in aluminum alloys.

Nature communications·2025
Same author

From Grotthuss Transfer to Conductivity: Machine Learning Molecular Dynamics of Aqueous KOH.

The journal of physical chemistry. B·2025
Same author

2D Carbon Phosphide for Trapping Sulfur in Rechargeable Li-S Batteries: Structure Design and Interfacial Chemistry.

ACS applied materials & interfaces·2024
Same author

Computational Exploration of Adsorption-Based Hydrogen Storage in Mg-Alkoxide Functionalized Covalent-Organic Frameworks (COFs): Force-Field and Machine Learning Models.

ACS applied materials & interfaces·2024
Same author

Ciprofloxacin and Azithromycin Antibiotics Interactions with Bilayer Ionic Surfactants: A Molecular Dynamics Study.

ACS omega·2024

Related Experiment Video

Updated: Jul 12, 2025

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
08:48

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Published on: April 28, 2022

1.8K

Lysozyme stability in various deep eutectic solvents using molecular dynamics simulations.

Akshatha Hebbar1, Poulumi Dey2, Anoop Kishore Vatti1

  • 1Department of Chemical Engineering, Manipal Institute of Technology (MIT), Manipal Academy of Higher Education (MAHE), Manipal, India.

Journal of Biomolecular Structure & Dynamics
|November 1, 2023
PubMed
Summary

Deep eutectic solvents (DESs) can stabilize proteins like lysozyme. Molecular dynamics simulations show DESs offer a more rigid protein structure than water, enhancing stability for industrial applications.

Keywords:
Lysozymedeep eutectic solventsmolecular dynamics simulationsprotein-DESs interactionsstability

More Related Videos

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

28.3K
Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
09:42

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

9.0K

Related Experiment Videos

Last Updated: Jul 12, 2025

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
08:48

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Published on: April 28, 2022

1.8K
How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

28.3K
Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
09:42

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

9.0K

Area of Science:

  • Biochemistry
  • Computational Chemistry
  • Materials Science

Background:

  • Proteins, including enzymes and therapeutic agents, are susceptible to denaturation from industrial stresses.
  • Deep eutectic solvents (DESs) are emerging as potential stabilizers for proteins.

Purpose of the Study:

  • To investigate the impact of various choline chloride-based DESs on the structural stability of a model protein, lysozyme.
  • To compare protein behavior in DESs versus water using molecular dynamics simulations.

Main Methods:

  • Molecular dynamics simulations of lysozyme in water and six different DESs.
  • Analysis of root mean square deviation (RMSD), root mean square fluctuations (RMSF), radius of gyration, and end-to-end distance.
  • Assessment of protein-solvent interactions via hydrogen bonding and radial distribution functions (RDF).

Main Results:

  • Most DESs resulted in a more rigid lysozyme structure compared to water.
  • Reline and levuline promoted protein compactness, while oxaline caused expansion.
  • Increased surface area exposure in malicine and oxaline indicated greater residue interaction with the solvent.

Conclusions:

  • Certain DESs, particularly reline, levuline, and polyol-based ones, can stabilize lysozyme.
  • DESs offer a promising alternative to water for maintaining protein integrity under stress, despite minor structural changes.