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Multimodal Optical Imaging Platform for Studying Cellular Metabolism
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Towards multimodal cellular imaging: optical and X-ray fluorescence.

Marcus E Graziotto1, Clinton J Kidman2, Liam D Adair1,3,4

  • 1School of Chemistry, The University of Sydney, Sydney, NSW, 2006, Australia. elizabeth.new@sydney.edu.au.

Chemical Society Reviews
|November 1, 2023
PubMed
Summary

Multimodal imaging combines optical fluorescence microscopy (OFM) and X-ray fluorescence microscopy (XFM) to reveal cellular molecular interactions. This powerful combination enhances understanding of health, disease, and therapeutic effects in biological systems.

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Area of Science:

  • Molecular imaging
  • Cellular biology
  • Biophysics

Background:

  • Imaging techniques provide unique chemical insights into cellular environments.
  • Multimodal imaging maximizes information from single samples by combining techniques.
  • Optical fluorescence microscopy (OFM) offers specificity and sensitivity.

Purpose of the Study:

  • To review recent studies combining OFM and X-ray fluorescence microscopy (XFM).
  • To demonstrate the application of combined OFM/XFM in diverse cellular systems.
  • To highlight the potential of this multimodal approach for understanding disease.

Main Methods:

  • Utilizing a single probe detectable by multiple imaging modalities.
  • Integrating OFM for high specificity and sensitivity.
  • Employing XFM for quantitative and element-specific analysis.

Main Results:

  • Combined OFM/XFM provides a comprehensive understanding of cellular molecular interactions.
  • This approach yields greater insight into how native elements and therapeutics impact cells.
  • Demonstrated applicability across a broad range of biological models.

Conclusions:

  • The synergistic combination of OFM and XFM significantly enhances biological imaging capabilities.
  • This multimodal strategy unlocks new knowledge regarding cellular function and disease states.
  • Future research can leverage this technique for deeper insights into complex biological systems.