Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry
Susy Daumoine1, Léa Hampe1, Emeric Limagne1
1Transfer Platform in Cancer Biology, Georges François Leclerc Cancer Center - UNICANCER, 1 rue du Professeur Marion, 21000 Dijon, France; UMR INSERM 1231, 7 Boulevard Jeanne d'Arc, 21000 Dijon, France; University of Burgundy-Franche Comté, Maison de l'université Esplanade Erasme, 21000 Dijon, France.
STAR Protocols
|November 1, 2023
Summary
This study details a flow cytometry protocol to analyze lymphocyte function in colorectal cancer patients. It enables simultaneous assessment of peripheral and intratumoral immune cells for better cancer research.
Area of Science:
- Immunology
- Oncology
- Biotechnology
Background:
- Assessing immune cell function is crucial for understanding cancer immunology.
- Current methods may not efficiently capture both peripheral and tumor-infiltrating immune cell profiles simultaneously.
Purpose of the Study:
- To present a novel protocol for the simultaneous flow cytometric analysis of peripheral and intratumoral lymphocyte function.
- To provide a standardized method for researchers studying colorectal cancer immunity.
Main Methods:
- The protocol involves simultaneous analysis of peripheral and intratumoral lymphocyte function using flow cytometry.
- Key steps include tumor digestion, pre-labeling of tumor-infiltrating leukocytes (TILs), and activation/labeling of total cells from both tumor and blood samples.
Main Results:
- The presented protocol allows for the concurrent evaluation of immune cell function from distinct patient sample types.
- This method facilitates a comprehensive understanding of the immune landscape in colorectal cancer.
Conclusions:
- This protocol offers a robust approach for simultaneous lymphocyte function analysis in colorectal cancer.
- It is a valuable tool for advancing research in cancer immunology and immunotherapy.
