Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry

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Updated: Jul 12, 2025

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
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Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Published on: January 7, 2019

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Susy Daumoine1, Léa Hampe1, Emeric Limagne1

  • 1Transfer Platform in Cancer Biology, Georges François Leclerc Cancer Center - UNICANCER, 1 rue du Professeur Marion, 21000 Dijon, France; UMR INSERM 1231, 7 Boulevard Jeanne d'Arc, 21000 Dijon, France; University of Burgundy-Franche Comté, Maison de l'université Esplanade Erasme, 21000 Dijon, France.

STAR Protocols
|November 1, 2023
PubMed

Keywords:
CancerFlow CytometryHealth SciencesImmunology

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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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