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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Jul 12, 2025

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

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Published on: November 13, 2017

Microarray fabrication techniques for multiplexed bioassay applications.

Roshan Tosh Aggarwal¹, Leyun Lai¹, Huiyan Li¹

¹School of Engineering, University of Guelph, Guelph, Ontario, N1G2W1, Canada.

Analytical Biochemistry

|November 1, 2023

Summary

This summary is machine-generated.

This review critically examines microarray fabrication methods for bioassays. It covers printing, microlithography, and microfluidics, offering insights for selecting techniques based on specific applications.

Keywords:

Bioassays High-throughput Microarray fabrication Multiplex Nanoarray

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Area of Science:

Biotechnology
Bioengineering
Materials Science

Background:

Microarrays enable high-throughput bioassays using biomolecule-spotted micro-spots.
Applications in drug discovery and diagnostics require diverse microarray features (shape, size, chemistry).
Micro/nano-fabrication is crucial for tailoring microarrays to specific assay needs.

Purpose of the Study:

To critically review fabrication methods for microarrays in recent bioassay studies.
To summarize common techniques and address literature gaps.
To provide insights for selecting appropriate fabrication methods.

Main Methods:

Review of microarray printing techniques.
Analysis of various microlithography methods.
Examination of microfluidics-based microarray fabrication.

Main Results:

Common microarray fabrication techniques were summarized.
Recent examples of microarray fabrication and bioassay applications were discussed.
Performance comparison based on different applications was evaluated.

Conclusions:

Understanding fabrication methods is essential for effective microarray design.
The review addresses literature gaps and guides technique selection.
Future directions and current challenges in microarray fabrication were outlined.