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Updated: Jul 12, 2025

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The Effect of Resveratrol in Sirt1/CST Pathway to Inhibit TNF-α Induced Inflammatory Response in Rat Primary

Xu-Meng Chen1, Yi-Jie Guo1, Hui-Wen Ling1

  • 1School of Pharmacy, Hunan University of Chinese Medicine.

Biological & Pharmaceutical Bulletin
|November 1, 2023
PubMed

Insights

Resveratrol, a natural compound, effectively reduces inflammation in rheumatoid arthritis fibroblast cells. It works by activating the Sirtuin1/cortistatin pathway, offering a potential natural therapy for the condition.

Area of Science:

  • Biochemistry
  • Immunology
  • Pharmacology

Background:

  • Rheumatoid arthritis significantly impacts quality of life, with current treatments having limitations.
  • Natural compounds like resveratrol are being explored for their therapeutic potential in rheumatoid arthritis.
  • Resveratrol, derived from Polygonum cuspidatum, is a promising polyphenol for intervention.

Purpose of the Study:

  • To investigate the role and mechanism of resveratrol in inhibiting inflammatory responses.
  • To examine resveratrol's effect on fibroblast-like synoviocytes in a rheumatoid arthritis model.
  • To elucidate the involvement of the Sirtuin1/cortistatin pathway in resveratrol's anti-inflammatory action.

Main Methods:

  • Utilized rat primary fibroblast-like synoviocytes.
  • Induced inflammation using Tumor Necrosis Factor-alpha (TNF-α).
  • Employed Sirtuin1 inhibitor (Selisistat/EX527) and cortistatin small interfering RNA (siRNA) for mechanistic studies.

Main Results:

  • Resveratrol inhibited TNF-α-induced Interleukin (IL)-1β and IL-6 secretion in a time- and dose-dependent manner.
  • Resveratrol upregulated Sirtuin1 and cortistatin mRNA and protein expression.
  • Inhibition of Sirtuin1 or cortistatin attenuated resveratrol's anti-inflammatory effects, confirming pathway involvement.

Conclusions:

  • Resveratrol demonstrates significant anti-inflammatory effects in fibroblast-like synoviocytes.
  • The mechanism involves the upregulation of the Sirtuin1/cortistatin pathway.
  • Resveratrol presents a potential natural therapeutic agent for managing rheumatoid arthritis inflammation.
Keywords:
Sirtuin1cortistatininflammationresveratrolrheumatoid arthritis

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