Structure-seq of tRNAs and other short RNAs in droplets and in vivo
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Updated: Jul 12, 2025

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
Published on: February 2, 2024
McCauley O Meyer1, Saehyun Choi2, Christine D Keating3
1Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, United States; Center for RNA Molecular Biology, Pennsylvania State University, University Park, PA, United States.
Abstract:
There is a multitude of small (<100nt) RNAs that serve diverse functional roles in biology. Key amongst these is transfer RNA (tRNA), which is among the most ancient RNAs and is part of the translational apparatus in every domain of life. Transfer RNAs are also the most heavily modified class of RNAs. They are essential and their misregulation, due to mutated sequences or loss of modification, can lead to disease. Because of the severe phenotypes associated with mitochondrial tRNA defects in particular, the desire to deliver repaired tRNAs via droplets such as lipid nanoparticles or other compartments is an active area of research. Here we describe how to use our tRNA Structure-seq method to study tRNAs and other small RNAs in two different biologically relevant contexts, peptide-rich droplets and in vivo.
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