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Published on: November 26, 2018
ILC3: a case of conflicted identity
Ivan Koprivica1, Suzana Stanisavljević1, Dragica Mićanović1
1Department of Immunology, Institute for Biological Research "Siniša Stanković" - National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia.
Innate lymphoid cells type 3 (ILC3s) are crucial immune sentinels. This study highlights inconsistencies in ILC3 identification methods, urging for standardized definitions to advance immunity research.
Area of Science:
- Immunology
- Cell Biology
Background:
- Innate lymphoid cells type 3 (ILC3s) are key sentinels in mucosal immunity and homeostasis.
- ILC3s are increasingly recognized for their role in chronic inflammatory and autoimmune diseases.
Observation:
- Flow cytometry is the standard method for ILC3 detection and characterization.
- Significant discrepancies exist in the phenotypic definitions of human and mouse ILC3s across studies.
Findings:
- Various inclusion and exclusion markers are inconsistently applied for ILC3 identification.
- The paper analyzes the advantages and disadvantages of different markers used for ILC3 phenotyping.
- Methods for efficient isolation and propagation of ILC3s for in vitro and in vivo studies are considered.
Implications:
- Standardizing ILC3 definitions, isolation, and propagation protocols is essential.
- Uniformity will enable more consistent interpretation of ILC3 functions in immunity.
- This work aims to facilitate more conclusive research on ILC3s' role in health and disease.
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