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Related Concept Videos

Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

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Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
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DNA Bacteriophages01:26

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Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
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Viral Replication: Lytic Cycle01:20

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Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
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Viral Replication: Lysogenic Cycle01:16

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The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
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Lysogenic Cycle of Bacteriophages00:43

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In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
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Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
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Related Experiment Video

Updated: Jul 11, 2025

Following Cell-fate in E. coli After Infection by Phage Lambda
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Architecture of the bacteriophage lambda tail.

Chang Wang1, Jinsong Duan1, Zhiwei Gu1

  • 1State Key Laboratory of Membrane Biology, Beijing Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Beijing 100084, P.R. China.

Structure (London, England : 1993)
|November 2, 2023
PubMed
Summary
This summary is machine-generated.

Bacteriophage lambda

Keywords:
LamBMan-PTSbacteriophagebaseplate hubcaudoviralescryo-EMdelivery devicephage lambdasiphoviridaetail complex

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Area of Science:

  • Virology
  • Structural Biology
  • Molecular Biology

Background:

  • Bacteriophage lambda is a well-studied virus with a double-stranded DNA genome.
  • Its tail mediates host recognition and DNA delivery.
  • Understanding the tail's structure is crucial for comprehending viral infection mechanisms.

Purpose of the Study:

  • To determine the high-resolution structure of the bacteriophage lambda tail complex.
  • To elucidate the molecular organization of the lambda tail.

Main Methods:

  • Cryo-electron microscopy (cryo-EM) was employed to capture the tail structure.
  • Atomic-scale resolution was achieved for most component proteins.

Main Results:

  • A high-resolution structure of the bacteriophage lambda tail complex was obtained.
  • The molecular organization of the tail proteins was revealed at near-atomic detail.

Conclusions:

  • The determined structure provides unprecedented insight into the bacteriophage lambda tail.
  • This work advances our understanding of viral structure and function.