Changes in community structure and microbiological risks in a small stream after receiving treated shale gas...

Discharge of treated shale gas wastewater is becoming prevalent in the Sichuan Basin in China, and the resulting potential environmental impacts have raised concern. In this study, the responses of microbial community in the receiving water to discharge of treated shale gas wastewater were assessed during a two-year study period, covering two wet seasons and one dry season. The results showed that the discharge of treated shale gas wastewater had no significant effects on alpha diversity in the two wet seasons, but had significant effects in the dry season after 15 months of discharge. Obvious changes in microbial community structure were observed in all three seasons at the downstream sites near the wastewater outfall, as compared to the control site. Multimetric indices indicated that the impacts of wastewater discharge on microbial ecosystem occurred with the extension of the discharge period. Moreover, special attention was given to the microbiological risks associated with antibiotic resistance genes (ARGs), virulence factor genes (VFGs), and pathogenic antibiotic resistant bacteria (PARBs) in the dry season in sediments of the receiving water. At downstream sites near the outfall, five subtypes of ARGs and seven VFGs showed a significant increase in relative abundance. Forty-two PARBs carrying ARGs and VFGs were detected, and three PARBs (Pseudomonas aeruginosa, Pseudomonas stutzeri and Pseudomonas fluorescens) increased obviously in relative abundance at the downstream site near the outfall. In conclusion, long-term wastewater discharge had effects on the microbial community, and limited microbiological risks existed in the receiving waters.