Metal organic layers enabled cell surface engineering coupling biomembrane fusion for dynamic membrane proteome...

Area of Science:

• Cell Biology
• Proteomics
• Biochemistry

Background:

• Understanding the membrane proteome is crucial for cellular processes.
• Current methods face challenges in selectivity and deep coverage of membrane proteins.
• Analyzing membrane proteins in living cells requires advanced techniques.

Purpose of the Study:

• To develop a novel method for comprehensive membrane proteome analysis in living cells.
• To map the plasma membrane and organelle membranes with high specificity and coverage.
• To investigate membrane protein modifications and dynamics during cellular events like apoptosis.

Main Methods:

• Developed a cell surface engineering coupled biomembrane fusion method.
• Utilized the interaction between 2D metal-organic layers and phospholipid bilayers.
• Integrated N-phosphoproteome analysis and quantitative proteomics.

Main Results:

• Successfully mapped the HeLa membrane proteome with 77% specificity and 5087 proteins.
• Enriched membrane proteins in their native phospholipid bilayer environment.
• Identified N-phosphorylation modifications and differential proteins during apoptosis, linked to mitochondrial dynamics and ER homeostasis.

Conclusions:

• The new method offers a simple, robust strategy for efficient membrane proteome analysis.
• Provides a reliable platform for studying membrane dynamics and cell events.
• Expands the application of metal-organic layers in biological research.