Detection of hapten erythrocyte conjugates in vitro and in vivo

The studies presented demonstrate that immunofluorescent techniques are capable of detecting picryl chloride erythrocyte protein conjugates formed in vitro and in vivo, following infusion of picryl chloride into normal, sensitized and tolerant guinea pigs. These experiments and the finding that picryl erythrocyte stromata prepared in vitro, and in vivo from infused recipients, have the capacity fo sensitize fresh animals, support the view that infused picryl chloride conjugates with the red cell membrane in vivo.