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Updated: Jul 11, 2025

Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds
Published on: May 5, 2016
Differentiation of embryonic stem cells into lung-like cells using lung-derived matrix sheets
Tomotaka Kitamura1, Masayasu Misu1, Masahide Yoshikawa1
1Department of Pathogen, Infection and Immunity, Nara Medical University, Kashihara, Nara, Japan.
Abstract:
Various extracellular matrix (ECM) in the lungs regulate tissue development and homeostasis, as well as provide support for cell structures. However, few studies regarding the effects of lung cell differentiation using lung-derived ECM (LM) alone have been reported. The present study investigated the capability of lung-derived matrix sheets (LMSs) to induce lung cell differentiation using mouse embryonic stem (ES) cells. Expressions of lung-related cell markers were significantly upregulated in ES-derived embryoid bodies (EBs) cultured on an LMS for two weeks. Moreover, immunohistochemical analysis of EBs grown on LMSs revealed differentiation of various lung-related cells. These results suggest that an LMS can be used to promote differentiation of stem cells into lung cells.

