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Updated: Jul 11, 2025
Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies
Published on: February 15, 2021
Protocol for the expansion of mouse immune effector cells for in vitro and in vivo studies
Thomas Look1, Hanna Meister1, Michael Weller2
1Department of Neurology, Clinical Neuroscience Center, University of Zurich, 8091 Zurich, Switzerland.
Abstract:
Reproducible and efficient expansion of different immune effector cells is required for pre-clinical studies investigating adoptive cell therapies against cancer. Here, we provide a protocol for the rapid expansion of mouse T cells, natural killer (NK) cells, and bone-marrow-derived macrophages (BMDMs). We describe steps for αCD3/αCD8 plate coating, isolating splenocytes, and expanding T cells and NK cells. Further, we detail procedures for bone marrow isolation and BMDM differentiation.
