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Updated: Jul 11, 2025

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis
Published on: December 16, 2016
Does water column stratification influence the vertical distribution of microplastics?
Kuddithamby Gunaalan1, Rodrigo Almeda2, Alvise Vianello3
1National Institute of Aquatic Resource, Technical University of Denmark, Denmark; Department of the Built Environment, Aalborg University, Denmark.
Microplastic pollution is widespread in marine waters, with similar concentrations found from surface to deep levels. This study found no impact of water density on microplastic distribution despite strong stratification in the Kattegat/Skagerrak.
Area of Science:
- Marine pollution
- Environmental science
- Oceanography
Background:
- Microplastic (MP) pollution is a pervasive global issue affecting all marine environments.
- Limited data exists on the sub-surface abundance and vertical distribution of microplastics.
- Water column stratification may influence microplastic distribution due to density differences.
Purpose of the Study:
- To investigate the vertical distribution of microplastics in the Kattegat/Skagerrak.
- To assess the relationship between microplastic abundance and water column structure.
- To identify dominant microplastic polymer types and sizes at different depths.
Main Methods:
- Water samples were collected from various depths at nine sites using plastic-free pump-filter devices.
- CTD measurements determined water column stratification and pycnocline depth.
- Microplastic concentrations, polymer types, sizes, and densities were analyzed.
Main Results:
- Microplastic concentrations ranged from 13 to 157 MP m⁻³ across all depths, with no significant difference between surface, mid, and deep waters.
- Polyester, polypropylene, polyethylene, and polystyrene were the dominant polymers.
- Approximately 94% of microplastics were between 11-300 μm, with 68% being high-density polymers.
Conclusions:
- Microplastics are ubiquitous throughout the marine water column in the Kattegat/Skagerrak.
- Water density and stratification did not significantly impact the vertical distribution of microplastics in this study.
- Findings highlight the widespread presence of microplastics, irrespective of water mass density variations.
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