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Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells
Published on: January 23, 2018
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A facile method to generate cerebral organoids from human pluripotent stem cells
Susan Simorgh1,2,3, Seyed Ahmad Mousavi2, San Kit To4
1Department of Developmental Biology, School of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran.
EXCLI Journal
|November 6, 2023
Summary
Human cerebral organoids (COs) generated from human pluripotent stem cells (hPSCs) mimic early brain development. This optimized protocol yields functional neural networks, advancing neurological research.
Area of Science:
- Neuroscience
- Developmental Biology
- Stem Cell Biology
Background:
- Human cerebral organoids (COs) offer a 3D model for studying neurological events.
- Neural induction from human pluripotent stem cells (hPSCs) is critical for CO generation.
- Signaling pathways in cell culture media influence hPSC differentiation.
Purpose of the Study:
- To optimize CO generation by modulating signaling cues from cell culture media.
- To develop a facile protocol for generating human cerebral organoids.
- To create a reliable platform for studying human brain development.
Main Methods:
- Applied a combination of small molecules and growth factors during hPSC culture.
- Utilized histological analysis for tissue organization assessment.
- Performed single-nucleus transcriptome profiling and electrophysiological recordings (patch clamp, Ca2+ dynamics).
Main Results:
- Generated COs recapitulate neural progenitor zones and early cortical layers.
- COs contain diverse neuronal and glial cell types, confirmed by transcriptomics.
- COs exhibit functional neural network activity via patch clamp and Ca2+ imaging.
Conclusions:
- The developed method provides a facile protocol for generating human cerebral organoids.
- These COs accurately mimic key features of the developing human brain.
- This platform advances research into neurological processes and human brain development.
