Related Experiment Video
Updated: Jul 11, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
[Nutrition and microbiota]
Francisco José Tinahones Madueño1
1Servicio de Endocrinología y Nutrición. Hospital Universitario Virgen de la Victoria.
Introduction:
The microbes that reside in our human body make up our microbiota, and their genes are known as the microbiome. The gut microbiota is involved in a wide variety of functions. At present there is considerable evidence indicating that in the last 60 years there has been an important change in the composition of our microbiota. Dietary changes have been shown to have important effects on the microbiota in a very short space of time. The Mediterranean diet pattern causes changes in the microbiota towards a healthier profile. The changes induced by the Mediterranean diet could be explained, to a large extent, by its richness in polyphenols.
Related Concept Videos
The Roles of Bacteria and Fungi in Plant Nutrition
Anatomy of the Intestines
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
Microbial Nutrition
Bacterial Flora of the Large Intestine
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
Microorganisms in Agriculture and Food industry
Fungal Phylum Microsporidia

