Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Jiedu Huoxue decoction inhibits cardiomyocyte apoptosis via PTEN/AKT/GSK3β-mediated mitochondrial dynamics in myocardial infarction: an integrative study of network pharmacology, transcriptomics and molecular docking.

Chinese medicine·2026
Same author

Study on the Properties of Nano-CeO<sub>2</sub>/Polyurea-Based Gel Grease for Electric Motor Bearings.

Gels (Basel, Switzerland)·2026
Same author

Age influences serum immune indices and gut microbiota composition in adult broilers.

Frontiers in microbiology·2026
Same author

SPARC Drives Tubulointerstitial Fibrosis through Regulating the CBP-DOT1L Pathway.

International journal of biological sciences·2026
Same author

Fructose 1-phosphate inhibits mannose phosphate isomerase to suppress hepatocellular carcinogenesis.

Signal transduction and targeted therapy·2026
Same author

Combining ultra-high pressure with propylene glycol and trehalose to inhibit co-elution of off-flavor components during liver protein extraction.

Food chemistry·2026

Related Experiment Video

Updated: Jul 11, 2025

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
11:26

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

13.8K

Keeping an Eye Out for Autophagy in the Cornea: Sample Preparation for Single-Cell RNA-Sequencing.

Han Peng1, Nihal Kaplan1, Min Liu1

  • 1Department of Dermatology, Northwestern University, Chicago, IL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 6, 2023
PubMed
Summary

Single-cell RNA-sequencing reveals autophagy

Keywords:
Beclin1 heterozygous mouseCell cluster identificationDissection of corneaEnzyme digestionSingle-cell suspension

More Related Videos

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments
09:12

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

Published on: October 4, 2021

3.3K
Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
09:49

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Published on: December 3, 2019

10.9K

Related Experiment Videos

Last Updated: Jul 11, 2025

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
11:26

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

13.8K
Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments
09:12

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

Published on: October 4, 2021

3.3K
Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
09:49

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Published on: December 3, 2019

10.9K

Area of Science:

  • Ophthalmology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Single-cell RNA-sequencing (scRNA-seq) enables gene expression profiling of individual cells.
  • This technique is valuable for studying rare cell populations, including stem cells.
  • Autophagy plays a role in cellular function and homeostasis.

Purpose of the Study:

  • To utilize scRNA-seq to analyze the limbal epithelial stem cell population.
  • To investigate the role of autophagy in limbal epithelial stem cell function.
  • To provide a protocol for scRNA-seq analysis of limbal tissues.

Main Methods:

  • Isolation of viable single-cell suspensions from limbal/corneal tissues.
  • Application of scRNA-seq to characterize cell populations.
  • Utilizing Beclin1 heterozygous mice as a model for autophagy deficiency.

Main Results:

  • scRNA-seq successfully profiled the limbal epithelial stem cell population.
  • The study identified specific gene expression patterns related to autophagy in these cells.
  • A detailed protocol for tissue processing and data analysis was established.

Conclusions:

  • scRNA-seq is effective for characterizing limbal epithelial stem cells.
  • Autophagy's contribution to limbal stem cell function can be elucidated using this approach.
  • The developed protocol facilitates further research into ocular surface stem cell biology.