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Updated: Jul 11, 2025

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
Published on: October 31, 2012
Generation of an induced pluripotent stem cell line (SHETi004-A) from a Chinese Han child with left bundle branch
Tingxia Wang1, Shan Hou1, Juan Chen1
1Department of Cardiology, Shanghai Children's Hospital, School of medicine, Shanghai Jiaotong University, No. 355 Luding Road, Shanghai, 200062, China.
Abstract:
Desmin (DES) is an important intermediate filament protein associated with the extrasarcomeric cytoskeleton and cellular function that was first reported to be associated with cardiac conduction disease and cardiomyopathy in 1998. We generated an induced pluripotent stem cell (iPSC) line from the left bundle branch block (LBBB) patient's peripheral blood mononuclear cells using Sendai virus-mediated reprogramming. The iPSCs exhibited stable amplification, expressed pluripotent markers, and spontaneously differentiated into three layers in vitro. Additionally, it showed a normal diploid karyotype and maintained the pathogenic mutation in DES. Hence, the iPSC line provided a platform for exploring LBBB mechanisms associated with DES mutations.
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