Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Oogenesis and germinal bed morphology of the brown anole (A. sagrei).

Developmental dynamics : an official publication of the American Association of Anatomists·2026

Same author

A long non-coding RNA Leat1 mediates the hormone responsiveness of EfnB2 during male urogenital development.

Communications biology·2025

Same author

Poly(A) probe HCR RNA-FISH specifically marks pyriform nurse cells in the brown anole lizard ovary.

Developmental biology·2025

Same author

Oogenesis and germinal bed morphology of the brown anole (<i>A. sagrei</i>).

bioRxiv : the preprint server for biology·2025

Same author

Dysregulated mitochondrial energy metabolism drives the progression of mucosal field effects to invasive bladder cancer.

The Journal of pathology·2025

Same author

WNT7A.

Differentiation; research in biological diversity·2025

Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025

Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025

Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025

Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025

Same journal

The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

Cold Spring Harbor protocols·2025

Same journal

Insights from the Study of B<i>-</i>Cell Epitopes of a Microbial Pathogen by Phage Display.

Cold Spring Harbor protocols·2025

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 11, 2025

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

Published on: November 20, 2018

Special Breeding Techniques for Use in Mouse Mutation Analysis.

Virginia E Papaioannou¹, Richard R Behringer²

¹Department of Genetics and Development, Columbia University Medical Center, New York, New York 10032, USA vep1@columbia.edu.

Cold Spring Harbor Protocols

|November 6, 2023

Summary

This summary is machine-generated.

Specialized breeding techniques aid in analyzing gene mutations and their interactions. Complementation testing and genetic interaction studies help understand gene function and phenotypes.

More Related Videos

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Related Experiment Videos

Last Updated: Jul 11, 2025

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

Published on: November 20, 2018

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Area of Science:

Genetics
Molecular Biology

Background:

Understanding gene mutations and their interactions is crucial for deciphering biological processes.
Various breeding techniques and genetic analyses are employed to study gene function.

Purpose of the Study:

To detail specialized breeding techniques for mutation analysis.
To explain methods for investigating genetic interactions.

Main Methods:

Complementation testing to identify alleles.
Breeding strategies to produce and test conditional and hypomorphic alleles.
Approaches to analyze genetic interactions in gene families, biochemical pathways, and genes with similar phenotypes.

Main Results:

Specialized breeding techniques can reveal gene function and allelic relationships.
Complementation testing identifies potential alleles based on similar phenotypes.
Genetic interaction studies elucidate functional relationships between genes.

Conclusions:

Advanced breeding and genetic analysis are essential tools for mutation characterization.
Understanding genetic interactions provides insights into complex biological pathways and gene redundancy.
These methods facilitate the study of gene function, regulation, and evolutionary relationships.