Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

14.0K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.0K
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

5.6K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
5.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oogenesis and germinal bed morphology of the brown anole (A. sagrei).

Developmental dynamics : an official publication of the American Association of Anatomists·2026
Same author

A long non-coding RNA Leat1 mediates the hormone responsiveness of EfnB2 during male urogenital development.

Communications biology·2025
Same author

Poly(A) probe HCR RNA-FISH specifically marks pyriform nurse cells in the brown anole lizard ovary.

Developmental biology·2025
Same author

Oogenesis and germinal bed morphology of the brown anole (<i>A. sagrei</i>).

bioRxiv : the preprint server for biology·2025
Same author

Dysregulated mitochondrial energy metabolism drives the progression of mucosal field effects to invasive bladder cancer.

The Journal of pathology·2025
Same author

WNT7A.

Differentiation; research in biological diversity·2025
Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025
Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025
Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025
Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025
Same journal

The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

Cold Spring Harbor protocols·2025
Same journal

Insights from the Study of B<i>-</i>Cell Epitopes of a Microbial Pathogen by Phage Display.

Cold Spring Harbor protocols·2025
See all related articles

Related Experiment Video

Updated: Jul 11, 2025

Forward Genetic Approach to Uncover Stress Resistance Genes in Mice &#8212; A High-throughput Screen in ES Cells
15:40

Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells

Published on: November 11, 2015

8.2K

Strategies for Maintaining Mouse Mutations.

Virginia E Papaioannou1, Richard R Behringer2

  • 1Department of Genetics and Development, Columbia University Medical Center, New York, New York 10032, USA vep1@columbia.edu.

Cold Spring Harbor Protocols
|November 6, 2023
PubMed
Summary
This summary is machine-generated.

Learn essential mouse mutation naming and maintenance strategies. This guide covers genetic background selection, colony management, and specialized breeding techniques for efficient mutant analysis.

More Related Videos

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

28.9K
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
06:46

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

9.8K

Related Experiment Videos

Last Updated: Jul 11, 2025

Forward Genetic Approach to Uncover Stress Resistance Genes in Mice &#8212; A High-throughput Screen in ES Cells
15:40

Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells

Published on: November 11, 2015

8.2K
Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

28.9K
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
06:46

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

9.8K

Area of Science:

  • Genetics
  • Animal Models
  • Molecular Biology

Background:

  • Establishing and maintaining genetically modified mouse models is crucial for biological research.
  • New mutations require standardized nomenclature and careful management for reproducibility.

Purpose of the Study:

  • To provide comprehensive guidelines for naming new mutations in mice.
  • To offer practical advice on selecting appropriate genetic backgrounds for mutant mouse strains.
  • To detail methods for maintaining mutant colonies and producing homozygous mutants for analysis.

Main Methods:

  • Discussion of established nomenclature rules for genetic mutations.
  • Considerations for choosing mouse genetic backgrounds based on mutation origin and research goals.
  • Strategies for colony management, including perpetuating mutations and generating homozygous offspring.
  • Explanation of specialized breeding techniques, such as in vivo selection cassette deletion and balancer chromosome use.
  • Addressing challenges like infertility in heterozygotes and the application of assisted reproductive technologies.

Main Results:

  • A framework for naming new mouse mutations is presented.
  • Guidance on genetic background selection is provided, considering mutation origin and analysis plans.
  • Methods for maintaining mutant colonies and producing homozygous mice are detailed.
  • Specialized breeding techniques, including in vivo selection cassette deletion and balancer chromosome maintenance, are discussed.
  • Assisted reproductive techniques are recommended for cases of heterozygote infertility.

Conclusions:

  • Standardized naming and strategic genetic background selection are vital for successful mouse mutant research.
  • Effective colony management and specialized breeding techniques ensure the efficient propagation and analysis of mutations.
  • Addressing potential issues like infertility with assisted reproduction preserves valuable mutant lines.