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Updated: Jul 11, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
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Draft genome sequence of a highly proteolytic Staphylococcus aureus USA300 isolate from human urine

Jessica K Jackson1, Sarah J Kennedy1, Emily A Felton1

  • 1Department of Molecular Biosciences, University of South Florida, Tampa, Florida, USA.

Microbiology Resource Announcements
|November 7, 2023
PubMed

Insights

We sequenced the genome of Staphylococcus aureus TGH337, a hyper-proteolytic USA300 strain from human urine. This provides insights into virulence factors contributing to Staphylococcus aureus infections.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Secreted proteases of Staphylococcus aureus are vital virulence factors during infection.
  • The USA300 strain is a significant cause of human disease.

Purpose of the Study:

  • To present the draft genome sequence of Staphylococcus aureus TGH337.
  • To characterize a hyper-proteolytic USA300 strain isolated from human urine.

Main Methods:

  • Genome sequencing of Staphylococcus aureus TGH337.
  • Bioinformatic analysis of the obtained genome sequence.

Main Results:

  • The draft genome sequence of Staphylococcus aureus TGH337 was successfully generated.
  • The strain exhibits hyper-proteolytic activity, consistent with its USA300 designation.

Conclusions:

  • The genome sequence provides a valuable resource for studying Staphylococcus aureus virulence.
  • Understanding the genetic basis of hyper-proteolysis in this strain can inform therapeutic strategies.
Keywords:
Staphylococcus aureusUSA300clinical isolateproteases

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