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Related Concept Videos

Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

2.5K
Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
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Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

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The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
693
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

3.7K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
3.7K
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.1K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.1K
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

654
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
654
Atomic Nuclei: Magnetic Resonance01:05

Atomic Nuclei: Magnetic Resonance

665
The number of nuclear spins aligned in the lower energy state is slightly greater than those in the higher energy state. In the presence of an external magnetic field, as the spins precess at the Larmor frequency, the excess population results in a net magnetization oriented along the z axis. When a pulse or a short burst of radio waves at the Larmor frequency is applied along the x axis, the coupling of frequencies causes resonance and flips the nuclear spins of the excess population from the...
665

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Related Experiment Video

Updated: Jul 11, 2025

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy
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A Data Deposition Platform for Sharing Nuclear Magnetic Resonance Data.

Matthew Pin1, Ella F Poynton1, Tamara Jordan1

  • 1Department of Chemistry, Simon Fraser University, 8888 University Drive, Burnaby, BC V5A 1S6, Canada.

Journal of Natural Products
|November 7, 2023
PubMed
Summary
This summary is machine-generated.

Nuclear magnetic resonance (NMR) data are often lost due to poor deposition practices. A new, user-friendly system is needed to improve data sharing and advance scientific discovery.

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Area of Science:

  • Chemistry
  • Biochemistry
  • Data Science

Background:

  • Nuclear magnetic resonance (NMR) data are crucial for scientific discovery but are rarely deposited in open databases.
  • Current methods of reporting NMR data (images, tables, lists) are insufficient and lack standardization, hindering data accessibility.
  • Limited access to NMR data impedes compound dereplication and the development of data-driven discovery tools, impacting fields like natural products research.

Purpose of the Study:

  • To address the challenges of NMR data deposition and accessibility.
  • To propose a streamlined and user-friendly mechanism for depositing and distributing NMR data.
  • To support the increasing requirement for data submission in scientific publications, such as in the Journal of Natural Products (JNP).

Main Methods:

  • Analysis of existing NMR data deposition practices and databases.
  • Identification of limitations in current data reporting formats and interfaces.
  • Conceptualization of a new system designed for ease of use and comprehensive data capture.

Main Results:

  • Existing NMR databases are often not optimized for natural products data or have complex deposition processes.
  • Current data reporting methods fail to capture the full information content of raw NMR data.
  • A significant need exists for a standardized, accessible platform for NMR data sharing.

Conclusions:

  • Improved NMR data accessibility is essential for advancing scientific knowledge and enabling data-driven research.
  • A user-friendly deposition system is required to encourage data sharing and comply with new journal mandates.
  • Facilitating FAIR data principles for NMR data will accelerate discovery in chemistry and related fields.