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Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
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Dual stop codon suppression in mammalian cells with genomically integrated genetic code expansion machinery

Birthe Meineke1, Johannes Heimgärtner1, Rozina Caridha1

  • 1Science for Life Laboratory, Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Genome Biology, 17165 Stockholm, Sweden; Ming Wai Lau Centre for Reparative Medicine, Stockholm Node, Karolinska Institutet, 17165 Stockholm, Sweden.

Cell Reports Methods
|November 7, 2023
PubMed

Keywords:
CP: BiotechnologyCP: Molecular biologyGenetic code expansionamber suppressionbioorthogonal labelingclick chemistrymammalian cell culturenon-canonical amino acidsrandom genomic integrationstop codon suppressionunnatural amino acids

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